Bifidus factor beer
A beer and hop technology, applied in the field of functional beer preparation, can solve the problems of lack of bifidobacteria cell component metabolites, increase the cost of beer, inability to decompose and utilize, etc., so as to enhance the immune function of the body, prevent and treat liver diseases, and strengthen the ecology. effect of effect
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Embodiment 1
[0022] 1.1 Raw materials: malt, commercially available rice or corn, granular hops.
[0023] 1.2 strains
[0024] 1.2.1 Bifidobacterium (Bifidobacterium, 86321): After hundreds of generations of oxygen-resistant acclimation, it has a certain tolerance to oxygen, but its biological characteristics remain unchanged. The bacteria are preserved in a refrigerator at 4°C by freeze-drying , take it out and resuscitate just before use.
[0025] 1.2.2 Saccharomyces cerevisiae (CICC1700): purchased from the Microbial Culture Collection Center of China Institute of Food and Fermentation Industry.
[0026] Process flow:
[0027] 2.1 Grinding raw materials
[0028] 2.1.1 Malt humidification and crushing Spray water mist at 60°C on the malt to increase the moisture content of the whole grain of malt by 1.5%. The sprayed water mist is fully mixed with the malt, and the humidification time is 2 minutes.
[0029] 2.1.2 Dry crushing of rice The lower the moisture, the finer the crushing and...
Embodiment 2
[0067] 1.1 Raw materials: malt, commercially available rice or corn, granular hops.
[0068] 1.2 strains
[0069] The fusant obtained by cell fusion of Bifidobacterium and Saccharomyces cerevisiae is used as a fermentation strain after regenerating the cell wall, and the strain is preserved in a refrigerator at 4°C by freeze-drying method, and taken out and resuscitated before use.
[0070] Process flow:
[0071] 2.1 Grinding raw materials
[0072] 2.1.1 Malt humidification and crushing Spray water mist at 60°C on the malt to increase the moisture content of the whole grain of malt by 1.5%. The sprayed water mist is fully mixed with the malt, and the humidification time is 2 minutes.
[0073] 2.1.2 The lower the water content of rice dry crushing, the finer the crushing, the better the effect; rice flour should not be stored for a long time to prevent heating and agglomeration.
[0074] 2.2 Preparation of wort
[0075] 2.2.1 The rice flour is gelatinized at 45°C and the ma...
Embodiment 3
[0093] 1.1 Raw materials: malt, commercially available rice or corn, granular hops.
[0094] 1.2 strains
[0095] The functional gene on the DNA of bifidobacteria was transformed into Saccharomyces cerevisiae through a plasmid vector to construct a genetically engineered strain, which was used as a fermentation strain. The strain was preserved in a refrigerator at 4°C by freeze-drying method, and it was taken out and revived immediately before use.
[0096] Process flow:
[0097] 2.1 Grinding raw materials
[0098] 2.1.1 Malt humidification and crushing Spray water mist at 60°C on the malt to increase the moisture content of the whole grain of malt by 1.5%. The sprayed water mist is fully mixed with the malt, and the humidification time is 2 minutes.
[0099] 2.1.2 The lower the water content of rice dry crushing, the finer the crushing, the better the effect; rice flour should not be stored for a long time to prevent heating and agglomeration.
[0100] 2.2 Preparation of w...
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