Rsf1010 derivative mob' plasmid containing no antibiotic resistance gene, bacterium comprising the plasmid and method for producing useful metabolites

1. The technology of RSF1010 and metabolites, applied in the field of mutant vectors, can solve the problems of restricting recombinant strains, not describing the stability of RSF1010 and its derivatives, and not being able to identify plasmid stability determinants, etc.
CN1973043AInactive Publication Date: 2007-05-30AJINOMOTO CO INC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
AJINOMOTO CO INC
Publication Date
2007-05-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

A Mob- plasmid having a RSF1010 replicon, comprising a gene coding for Rep protein and said plasmid has been modified to inactivate gene related to mobilization ability. The present invention also describes a bacterium having an ability to produce useful metabolites, comprising the plasmid and said bacterium lack active thymidylate synthase coded by thyA gene and thymidine kinase coded by tdk gene, and a method for producing useful metabolites, such as native or recombinant proteins, enzymes, L-amino acids, nucleosides and nucleotides, organic acid, vitamins, using the bacterium.
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Description

technical field

[0001] The present invention relates to mutant vectors and uses thereof, more particularly, the present invention relates to broad host range RSF1010 derived Mobs that do not contain antibiotic resistance genes - plasmid. The invention also relates to bacteria comprising said plasmid and methods of using said bacteria to produce useful metabolites. Background technique

[0002] RSF1010 is a well-known IncQ group plasmid that is mobilizable but not self-transmissible and has a remarkable ability to replicate in a broad bacterial host range, including most Gram-negative bacteria (Frey, J. and Bagdasarian, M. The molecular biology of IncQ plasmamids. In: Thomas, C.M. (eds.), Promiscuous Plasmids of Gram NegativeBacteria. Academic Press, London, 1989, p.79-94). The nucleotide sequence of the RSF1010 plasmid is known (Scholz, P. et al., Gene, 75(2), 271-288 (1989); accession number GenBank M28829, gi:152577) and this plasmid has been studied very thoroughly fun...

Claims

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