Method for selectively killing cells utilizing ultraviolet conversion luminous nano particles

A nanoparticle, cell-killing technology, applied in the fields of phototherapy, nanotechnology, luminescent materials, etc., can solve the problem of selective binding between ultraviolet up-conversion luminescent nanoparticles and target cells that has not yet been seen

Inactive Publication Date: 2007-06-13
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there have been no reports on the selective binding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Of the two types of cells mixed together, leukocytes and lymphocytes, the former is killed without harming the latter.

[0011] The specific method is as follows:

[0012] Firstly, the ultraviolet up-conversion luminescent nanoparticles are prepared. The method is: irradiating Zn soaked in ethanol with Nd:YAG pulsed laser 0.3 al 0.25 Pb 0.3 Li 0.098 Yb 0.1 T m 0.002 f 2.354 Glass, which is prepared using solid-state reactions. The particle size produced depends on the pulse time of the laser. For more detailed preparation information, refer to the Dutch magazine Optics Communications (No. 242, p. 215).

[0013] The following is to achieve the selective combination of the prepared nanoparticles and white blood cells: first, add the prepared nanoparticles to ammonia water to form a suspension, centrifuge the suspension at 5000 rpm for 10 minutes, remove the supernatant, and ultrasonicate at low temperature Shake for 15 minutes, then add glutaraldehyde...

Embodiment 2

[0016] Example 2: For tumor-bearing mice, tumor cells were killed without harming normal cells.

[0017] The specific method is as follows:

[0018] The ultraviolet up-conversion luminescent nanoparticles in Example 1 are still used. The surface treatment of the nanoparticles is also the same as Example 1.

[0019] The monoclonal antibody J591 was reacted with the treated nanoparticles at pH8 for two hours, and the amount of the antibody used was 20 times that of the nanoparticles (molar ratio). Then the product was centrifuged at high speed for 30 minutes, and then dispersed with PBS solution of pH7. After the above steps, nanoparticles with antibody J591 linked to the surface were prepared.

[0020] The aforementioned nanoparticles were injected into the tail veins of tumor-bearing mice. One hour later, the mice were irradiated with infrared light having a wavelength of 798 nanometers. Infrared light can penetrate several centimeters in biological tissue, so under the i...

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PUM

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Abstract

The present invention relates to a method capable of utilizing ultraviolet up-conversion granules to selectively kill cells, and is characterized by that it uses the ultraviolet up-conversion nano granules capable of sending ultraviolet light under the irradiation of infrared light or visible light, makes the antibody capable of implementing specific combination with target cell be connected on said nano granule surface and utilizes the characteristics of that said antibody and cell surface correspondent antigen can make selectively combination so as to implement selective combination of nano granules and target cells, then it adopts infrared light or ultraviolet light to irradiate the ultraviolet up-conversion luminous nano granules so as to kill the cells combined with ultraviolet light sent by said ultraviolet up-conversion granules.

Description

Technical field: [0001] The invention belongs to the technical field of methods for killing cells by ultraviolet light, and in particular relates to a method for selectively killing cells by using ultraviolet up-conversion luminescent nanoparticles. Background technique: [0002] The use of ultraviolet light to kill cells is a widely used technique. As pointed out on page 24 of "Medical Microbiology" (Edited by Qian Lisheng) published by Fudan University Press in 2003, this type of technology uses ultraviolet light to destroy the structure of DNA to kill cells. When using ultraviolet light to kill cells, ultraviolet light has a killing effect on cells within the irradiated range. But sometimes we want to kill only certain cells without affecting other cells around, but the existing ultraviolet light killing cell technology can not do this. In addition, due to the limited penetration ability of ultraviolet light, the application of ultraviolet light in killing cells in orga...

Claims

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Application Information

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IPC IPC(8): A61N5/06C09K11/08B82B1/00A61K51/02
Inventor 王冠中沈小双
Owner UNIV OF SCI & TECH OF CHINA
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