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Method of identifying Rock lily plant and its preparation

A technology of Selaginella and its preparations, which is applied in the field of identification of Selaginella plants and their preparations by thin-layer chromatography, and can solve the problems of extensive methods and the inability to propose the biflavonoids of Selaginella spica

Active Publication Date: 2007-06-27
GUANGZHOU BAIYUNSHAN PHARMA HLDG CO LTD BAIYUNSHAN PHARMA GENERAL FACTORY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a lot of Selaginella spicatum are used for research, but there are the following deficiencies in these two standards: 1) Using the method of boiling water, it is impossible to extract the amiflavones of Selaginella spica; The spots are all unknown, and the method is relatively extensive
And because the Pharmacopoeia only indicates that Selaginella jiangnanensis can be used as the medicinal material of Selaginella jiangnanensis tablets, in order to identify Selaginella jiangnanensis more effectively, it is not enough to identify the medicinal materials of Selaginella jiangnanensis only by existing technology

Method used

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  • Method of identifying Rock lily plant and its preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A. Preparation of the test product: each of the 11 kinds of plants to be tested weighed 2 g and added methanol 10 ml, sonicated for 10 min, filtered, the filtrate was evaporated to dryness, and the residue was dissolved by adding 2 ml of absolute ethanol to prepare a concentration of 1 g / ml. solution;

[0031] B. Preparation of the reference substance: Weigh 0.5 mg of the reference substance of Suixa chinensis biflavone into a volumetric flask, dissolve it with methanol and dilute to the 10ml mark, and make a 0.05 mg / ml reference substance solution;

[0032] C. Take 1ul of the test solution and the reference solution, and place them on the same polyamide thin-layer plate, with benzene-petroleum ether (60-90°C)-butanone-methanol (5:2:3:3) To develop with the developing agent, take it out and dry it in the air, then use sodium lauryl sulfate-n-heptane-n-butanol-water (3g:2:6:25) as the developing agent, spread it to about 8cm, take it out, and dry it in the air. Spray wi...

Embodiment 2

[0037] A, the preparation of test product: 11 kinds of plant whole plants to be tested each weighed 2g and added 40ml of 95% ethanol, cold soaked for 36 hours, filtered, and the filtrate was evaporated to dryness, and the residue was dissolved by adding 2ml of 95% ethanol to make a concentration of 0.5g / ml solution;

[0038] B. Preparation of the reference substance: Weigh 0.3 mg of the reference substance of Suixa chinensis biflavone into a volumetric flask, dissolve it with methanol and dilute to 10 ml scale, and make a 0.03 mg / ml reference substance solution;

[0039] C. Take 0.1ul of the test solution and 0.1ul of the reference solution, spot on the same silica gel G thin-layer plate, develop with toluene-ethyl formate-formic acid (5:4:1) as the developer, take it out and let it dry Dry it, spray it with 5% aluminum trichloride test solution, and inspect it under a UV lamp at 366nm;

[0040] The results are shown in Figure 3. At Rf=0.4, it can be observed that: 2. Selagi...

Embodiment 3

[0044] A, preparation of test product: 2 slices of Selaginella chinensis, each weighing 0.34g, pulverized, added 10ml of methanol, ultrasonically treated for 10min, filtered, the filtrate was evaporated to dryness, and the residue was added with absolute ethanol to dissolve and prepare a concentration of The solution of 2g / ml; The test sample preparation method of other 10 kinds of plant whole plants to be tested is the same;

[0045] B. Preparation of the reference substance: Weigh 0.6 mg of the reference substance of Suixa chinensis in a volumetric flask, dissolve and dilute to the 10ml scale with methanol, and make a 0.06mg / ml reference substance solution;

[0046] C. Take 1ul of the test solution and the reference solution, and place them on the same polyamide thin-layer plate, with benzene-petroleum ether (60-90°C)-butanone-methanol (5:2:3:3) To develop the developing agent, take it out and dry it, spray it with 5% aluminum trichloride test solution, and inspect it under ...

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Abstract

The present invention relates to method of identifying Rock lily plant and its preparation. The method includes alcohol extraction of Jiangnan Rock lily and positive contrast with amentoflavone. The present invention has the identification of Jiangnan Rock lily medicine material and preparation from other 10 kinds of confused Rock lily, including Yanzhou Rock lily, dark green Rock lily, etc. The quality control method has high specialization and high stability, and may be used in controlling the quality of Jiangnan Rock lily medicine material and its products, such as cosmetics, health product and medicine.

Description

technical field [0001] The invention relates to a method for identifying Selaginella plants and their preparations by thin-layer chromatography. Background technique [0002] Selaginella is a plant of the genus Selaginella of the family Selaginaceae, which is distributed all over the world. There are about 700 species, most of which are produced in the tropics and subtropics. It mostly grows on rock walls or in periodically dry soil. There are about 50 species in my country, distributed all over the country. Selaginaceae plants are ancient, specialized and evolutionary, and heterospores first appeared, which has special theoretical significance in evolution. It is generally believed that there is only one genus of Selaginellaceae plants in existence, namely Selaginella Beauv. So far, the research on the chemical constituents of Selaginella has mainly focused on the isolation, identification and qualitative analysis. It can be considered that biflavonoids and trehalose ar...

Claims

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Application Information

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IPC IPC(8): A61K36/14A61K8/97A61K9/20A61K9/48G01N30/90G01N33/15A61Q7/00A61Q19/00A61P31/04A61P17/00
Inventor 崔国华张瑞玲
Owner GUANGZHOU BAIYUNSHAN PHARMA HLDG CO LTD BAIYUNSHAN PHARMA GENERAL FACTORY
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