Optical probes and assays

a technology of optical probes and probes, applied in the field of optical probes and assays, can solve problems such as imprecise estimates, and achieve the effects of enhancing fluorescence anisotropy measurements, increasing rotational flexibility of optical probes, and reducing fluorescence polarization
US20030087328A1Inactive Publication Date: 2003-05-08VERTEX PHARMA SAN DIEGO LLC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
VERTEX PHARMA SAN DIEGO LLC
Publication Date
2003-05-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

This invention provides an optical probe useful as an optical probe or sensor of post translational type modifications, such as phosphorylation. The invention comprises a polypeptide moiety, which contains a recognition motif for a post translational type activity and a protease site, which is coupled to a probe moiety. Modification of the polypeptide, by the post translational type activity, results in a modulation of the rate at which a protease cleaves the polypeptide which is sensed by a measurable change in at least one optical property of the optical probe upon cleavage. The present invention also includes a recombinant nucleic acid molecule that encodes an optical probe and a vector and host cell or library of cells that include the recombinant nucleic acid molecule. The optical probe can be used in methods to determine whether a sample, including a cell or a sample from an organism, contains a post-translational type modification activity. Such methods can also be used to.determine whether a test chemical modulates the activity of a modifying activity, and thus can be used to identify therapeutic compositions. The identification of such therapeutic compositions can be automated using a system that includes an optical probe.
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Description

[0001] This application is a continuation of application U.S. Ser. No. 09 / 306,542, filed May 5, 1999, now allowed, the entire contents of which are hereby incorporated by reference herein.

[0002] The present invention relates generally to the fields of chemistry and biology. More particularly, the present invention relates to optical probes for post translational type modification activities, such as phosphorylation, and methods for their use.

[0003] Systems and methods for rapidly identifying chemicals with biological activity in samples, especially small liquid samples, is of particular relevance to the agrochemical and pharmaceutical fields. Various strategies are typically used to reduce processing times and associated costs of screening large numbers of chemical entities, including simplified assay design, automation, robotics and miniaturization of sample size. The advent of high throughput analysis and increasing use of miniaturized formats has led to the development of high de...

Claims

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