Protein purification

a technology of protein purification and purification chamber, applied in the field of protein purification, can solve the problems of difficult removal of subcellular fragments, formidable challenge of human use as a human therapeutic,
US20050215769A1Inactive Publication Date: 2005-09-29BREECE TIMOTHY +5
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
BREECE TIMOTHY
Publication Date
2005-09-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method for purifying proteins by Protein A chromatography is described which comprises removing contaminants by washing the solid phase with various intermediate wash buffers.
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Description

[0001] This is a non-provisional application claiming priority under 35 USC §119 to provisional application No. 60 / 354,579 filed Feb. 5, 2002, the entire disclosure of which is hereby incorporated by reference.BACKGROUND OF THE INVENTION

[0002] 1. Field of the Invention

[0003] This invention relates generally to protein purification. In particular, the invention relates to a method for purifying CH2 / CH3 region-containing proteins, such as antibodies and immunoadhesins, by Protein A affinity chromatography.

[0004] 2. Description of Related Art

[0005] The large-scale, economic purification of proteins is increasingly an important problem for the biotechnology industry. Generally, proteins are produced by cell culture, using either mammalian or bacterial cell lines engineered to produce the protein of interest by insertion of a recombinant plasmid containing the gene for that protein. Since the cell lines used are living organisms, they must be fed with a complex growth medium, containi...

Claims

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