Refined royal jelly

Abstract

The object of the present invention is to provide an allergen-reduced royal jelly, having less fear of causing allergic symptoms, obtained by reducing allergens and keeping the useful pharmacological actions of royal jelly, a process for producing the same, and its uses. The present invention solves the above object by providing an allergen-reduced royal jelly where the amount of water-soluble proteins is reduced to less than 50% (w / w) to the amount of total proteins; a process for producing the allergen-reduced royal jelly comprising the steps of separating a royal jelly into a precipitate and a supernatant comprising water-soluble proteins by adding water and centrifuging, collecting low molecular substances by eliminating high molecular substances comprising water-soluble proteins from the resulting supernatant using ultra-filtration or gel filtration chromatography, and mixing the resulting low molecular substances with the aforesaid precipitate; a composition comprising the allergen-reduced royal jelly; and the uses of the composition for foods, feeds, baits, pet foods, and cosmetics.

Description

TECHNICAL FIELD [0001] The present invention relates to an allergen-reduced royal jelly (Hereinafter, simply abbreviated as “ARRJ”), particularly, ARRJ where the amount of water-soluble proteins is reduced to less than 50% (w / w) to that of total proteins, a process for producing the same, and its uses. BACKGROUND ART [0002] Royal jelly is a milk-white humor, secreted from cephalic glands of worker honeybees and stored in a royal cell (a cell for queen bee) of honeycomb, and a jelly given as a feed to larva which grows to queen bee. Although its chemical composition is varied in some degree depending on its source and season, it has been reported that the composition is 65-75% (w / w) of water, 15-20% (w / w) of proteins, 10-15% (w / w) of carbohydrates, 1.7-6% (w / w) of fats, and 0.7-2% (w / w) of ashes. Further, royal jelly comprises organic acids represented by 10-hydroxy-2-decenoic acid, various vitamins and minerals. It has been reported that proteins of royal jelly includes water-solubl...

AI Technical Summary

Benefits of technology

[0005] The present invention was accomplished under these circumstances. The object of the present invention is to provide ARRJ having less fear of causing allergic symptom by reducing allergens and keeping the useful pharmacological actions of royal jelly, a process for producing the same, and the uses of a composition comprising ARRJ for foods, feeds, baits, pet foods, and cosmetics.

[0006] To solve the above object, the present inventors have extensively studied on a high quality ARRJ having less fear of causing allergic symptom by reducing allergens and keeping the useful pharmacological actions of royal jelly and a process for producing AARJ. As a result, the present inventors found that the most part of allergic substances, comprised in a fresh royal jelly, are water-soluble and contained in supernatant by the steps of admixing water with non-treated royal jelly (hereinafter, “non-treated royal jelly” may be simply abbreviated as “royal jelly” or “fresh royal jelly” in this specification. Further, it may be abbreviated as “RJ” in Tables.) as a material, separating the mixture into a supernatant and a precipitate by centrifugation, and measuring the allergic activity of those using a fresh royal jelly as a control. The present inventors also found as original knowledge that ARRJ, where the amount of water-soluble proteins is reduced to less than 50% (w / w) (hereinafter, “% (w / w)” is simply abbreviated as “%” in this specification) to the amount of total proteins and the amount of allergens is reduced, which keeps the useful pharmacological actions of fresh royal jelly, can be produced by a method constructed by combining the processes of admixing water with a fresh royal jelly and separating the mixture into a supernatant and a precipitate by centrifugation (hereinafter, the process is abbreviated as “process of water-fractionation” in this specification); collecting low molecular substances by eliminating high molecular substances comprising water-soluble proteins from the resulting supernatant using ultra-filtration or gel-filtration chromatography (hereinafter, the process is abbreviated as “process of high molecular substances elimination” in this specification); and mixing the resulting low molecular substances with the aforesaid precipitate (hereinafter, the process is abbreviated as “process of mixing” in this specification).

Problems solved by technology

However, since royal jelly contains allergic substances, it may cause an allergic symptom when it is consumed.

Although the allergic symptom varies depending on a person, in some cases, royal jelly causes a significant anaphylatic shock.

However, in the case of such royal jelly produced by the steps of precipitating allergic water-soluble proteins by isoelectric point precipitation and degrading the resulting precipitate by saccharide-degrading enzymes and protein degrading enzymes, the additional enzymes themselves would possibly be allergic substances.

Therefore, the above royal jelly has a disadvantage of not being preferable as royal jelly for keeping health.