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Isolation, identification and characterisation of strains with probiotic activity, from faeces of infants fed exclusively with breast milk

a technology of probiotic activity and faeces, which is applied in the field of isolate, identification and characterisation of strains with probiotic activity, from, can solve the problem that isolate strains do not meet the probiotic condition

Inactive Publication Date: 2013-10-24
HERO ESPANA S A ALCANTARILLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent is about new probiotic microorganisms that have been isolated from the feces of babies. These microorganisms have improved properties as probiotics and can benefit the host by improving intestinal balance. The patent also mentions the use of prebiotics, which are non-digestible ingredients that stimulate the growth of intestinal bacteria. Examples of prebiotics include inulin, oligofructosaccharides, and resistant starches. Overall, the patent aims to provide a solution for improving gut health and promoting well-being through the use of these new probiotic microorganisms and prebiotics.

Problems solved by technology

However, many of the isolated strains do not meet the probiotic condition since they are rarely or are not resistant to the digestive juices and many of them do not adhere to the intestinal epithelium.

Method used

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  • Isolation, identification and characterisation of strains with probiotic activity, from faeces of infants fed exclusively with breast milk
  • Isolation, identification and characterisation of strains with probiotic activity, from faeces of infants fed exclusively with breast milk
  • Isolation, identification and characterisation of strains with probiotic activity, from faeces of infants fed exclusively with breast milk

Examples

Experimental program
Comparison scheme
Effect test

example 1

Amplifying 16S-23S Intergenic Fragments

[0153]The intergenic segments of the selected strains were amplified, sequenced and the homology search was performed in the NCBI (BLAST) database which delivered the following results:

[0154]Lactobacillus rhamnosus 22A (CNCM I-4036) has a homology of 100% of a 579 bp fragment with:[0155]Lactobacillus rhamnosus isolate TS1[0156]Lactobacillus rhamnosus isolate PS1 16S

[0157]Lactobacillus paracasei 7 (CNCM I-4034) has a homology of 100% of a 512 bp fragment with:[0158]Lactobacillus casei ATCC 334

[0159]Bifidobacterium breve 15B (CNCM I-4035) has a homology of 99% of the 182 bp fragment 16s-23s intergenic space, with:[0160]Bifidobacterium breve (ITS), strain Y8[0161]Bifidobacterium longum (ITS), strain Y10

example 2

Aligning Sequenced Sections

[0162]The Clustalw program on-line tool (http: / / www.ebi.ac.uk / clustalw / ) was used to align the sequenced sections of the selected strains and controls.

[0163]The overall alignment of the sequences of rDNA 16S gene of the strains with the controls showed the existence of differences between the sequences of the controls and the samples L. rhamnosus 22A and L. paracasei 7, and a complete homology between the sample 7 and a selected sequence of L. paracasei is observed.

[0164]Relating to the selected sample β. breve 15B, a difference between the sequences of the controls and the sample B. breve 15B, and a homology of 100% between B. breve 15B and a selected sequence of B. breve is observed.

[0165]The overall alignment of the sequences of the intergenic space 16S-23S of the strain 15B and controls allowed observing a large difference between the sequence of the controls and the sample B. breve 15B.

[0166]In fact the sequencing of the 16S-23S intergenic space is un...

example 3

Taking and Processing the Samples

Taking of Samples.

[0167]Feces from exclusively breast-fed children between 2 and 4 months old were taken in anaerobic conditions at the clinic of the pediatrician J M, the inventor of this patent. Parents were asked to bring their children to the clinic first thing in the morning, the children was expected to have a bowel movement after stimulation and after the bowel movement the feces were collected in a sterile container by means of a plastic spoon which is adhered to its cover. Once the collection ended, the container with the sample was introduced in an anaerobic jar (Anaerojan®, Oxoid, Hampshire, United Kingdom) accompanied by a sachet generating anaerobic atmosphere (Anaerogen®, Oxoid, Hampshire, United Kingdom) and the jar was sealed hermetically and transported to the laboratory where the samples were processed in a time no longer than 2 hours.

Processing and Seeding the Samples

[0168]The samples to be analyzed can be handled once collected or...

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Abstract

The invention relates to probiotic micro-organisms isolated from feces of children exclusively fed with breast milk. Said microorganisms are used in the food or pharmaceutical industry, especially for use in infant formula milk, due to their probiotic properties which have beneficial effects on the health of those ingesting them. Said microorganisms consist of Lactobacillus rhamnosus HERO 22A (CNCM I-4036), Lactobacillus paracasei HERO 7 (CNCM I-4034) and Bifidobacterium breve HERO 15B (CNCM I-4035).

Description

TECHNICAL SECTOR OF THE INVENTION[0001]The general objective of this study is to isolate probiotic microorganisms for a subsequent use in the food and pharmaceutical industry, especially for using them in infant formula milk. Said microorganisms have high values of resistance to pH, bile salts and intestinal cell adhesion, they are therefore especially suitable for use in the aforementioned industries.BACKGROUND OF THE INVENTION[0002]Nutrition has had a very significant development which has changed its concept in the recent decades. Diet was previously considered as having the role of providing nutrients necessary to maintain the health condition, whereas today this concept has evolved into the idea that diet can contain foods which in addition to providing nutrition, promote health. This is the reason why the food industry has started to develop a large amount of products promoting health and wellbeing. In this area the line of functional foods has had a very significant developme...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N1/20A23L7/10A23L29/00A23L33/00
CPCC12N1/20A23V2002/00A23L33/40A23L33/135A61P1/00A61P11/06A61P3/02A61P3/04A61P31/00A61P37/04A61P3/10C12R2001/225C12N1/205C12R2001/01A23V2400/519A23V2400/175A23V2200/3204A61K35/745A61K35/747
Inventor VIEITES FERNANDEZ, JOSE MARIAMUNOZ QUEZADA, SERGIOLLAMAS COMPANY, INMACULADAMALDONADO LOZANO, JOSEROMERO BRAQUEHAIS, FERNANDOSUAREZ GARCIA, ANTONIO FRANCISCOGIL HERNANDEZ, ANGELGOMEZ LLORENTE, CAROLINABERMUDEZ BRITO, MIRIAM
Owner HERO ESPANA S A ALCANTARILLA