Method and System for Treatment of Biological Tissue
a biological tissue and treatment method technology, applied in the field of biological tissue treatment methods, can solve the problems of abnormal wall motion, myocardial cell death, and myocardial tissue that is no longer receiving adequate blood flow, and achieves the effects of promoting tissue survival, promoting tissue survival, and promoting neovascularization and regeneration
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example 1
[0149]Five (5) porcine hearts were obtained from young calves. After removal, the hearts were stored in a saline bath.
[0150]A first heart was removed from the bath. The thickness of the heart wall was determined to range from 4 mm to greater than 2 cm with an A scan ultrasound sensor.
[0151]A multi-needle injection system of the invention, such as illustrated in FIGS. 2 and 3 was provided and prepared for the injection procedure.
[0152]An ECM composition of the invention was also provided. The ECM composition comprised two components: an ECM (i.e. SIS) particulate derived from porcine intestines and a SIS gel. The SIS particulate comprised SIS material, which was cryogenically ground to a characterized particle size, and subsequently thawed and loaded into a syringe for delivery. The particulate size was in the range of 50-350 microns.
[0153]The SIS gel comprised SIS material that was cryogenically ground, subject to enzymatic digestion in acid, lyophilized, and reconstituted to a pred...
example 2
[0165]A young porcine was provided in which CHF had been induced via serial microsphere injections down the coronary arteries.
[0166]A multi-needle injection system of the invention, such as illustrated in FIGS. 2 and 3, was prepared for injection of an ECM composition of the invention.
[0167]An ECM composition, such as described in Example 1, was also provided. The composition mixture comprised approximately 4 cc of SIS gel was mixed with 6 cc of particulate SIS to derive an injectable ECM composition.
[0168]The injectable ECM composition was then transferred into the reservoirs of the injector apparatus.
[0169]The injector control system was similarly set to provide the following delivery parameters: two (2) equal pulses at 20 and 30 milliseconds and at pressures ranging from approximately 60-120 psi. The noted parameters provided an ECM composition delivery in the range of approximately 0.5-1.0 ml per pulse.
[0170]The heart of the porcine was then exposed. A B scan ultrasound sensor w...
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