PCR validation tubes
validation tube technology, applied in the field of methods for validating a polymerase chain reaction apparatus, can solve the problems of not using a standard method, unable to quantify the amount of starting material, and using a fluorescence signal obtained in a standard fluorimeter, etc., to achieve reusable and stable results
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[0032]FIG. 1 shows a reference standard 1 for use in a thermal cycler. The reference standard 1 is made by following the steps of:[0033](a) providing suitable thermoplastic monomers;[0034](b) mixing the thermoplastics monomers with a suitable fluorophore;[0035](c) carrying out a bulk polymerisation reaction to obtain a polymer comprising a fluorophore suspended substantially uniformly therein;[0036](d) drying the polymer comprising the suspended fluorophore;[0037](e) machining the dried polymer comprising the suspended fluorophore; and[0038](f) polishing the dried polymer comprising the suspended fluorophore.
[0039]In other words, the reference standard is produced by mixing a fluorophore or fluorescent dye with, for example, methyl methacrylate monomers and then polymerising the methyl methacrylate monomers using an initiator in a bulk polymerisation process using a suitable initiator, such as, for example, azo compounds or organic peroxides. The resultant material, which will be de...
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