Preparation and application of a kind of ferric oxide complex metal chelate mimetic enzyme

A technology of ferroferric oxide and composite metal, which is applied in the field of nanotechnology and analysis and detection, can solve the problems of complex analysis process, high analysis cost, and long analysis time, and achieve the effect of simple separation process, good selectivity, and high sensitivity

Active Publication Date: 2021-08-24
HANGZHOU DIANZI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, detecting H 2 o 2 Especially important, there are a variety of detection methods nowadays, such as spectrophotometry, electroanalysis, resonance light scattering analysis, chromatography, etc., but these methods have some disadvantages, such as high analysis cost, long analysis time, and analysis process. Complexity, low signal-to-noise ratio, low sensitivity, poor selectivity, etc., it is difficult to meet the domestic and foreign requirements for H 2 o 2 Further requirements for testing

Method used

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  • Preparation and application of a kind of ferric oxide complex metal chelate mimetic enzyme
  • Preparation and application of a kind of ferric oxide complex metal chelate mimetic enzyme
  • Preparation and application of a kind of ferric oxide complex metal chelate mimetic enzyme

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1, Fe 3 o 4 -Cu 2+ Preparation of mimetic enzyme

[0038] Step 1, weigh 2.162gFeCl 3 ·6H 2 O, 5.4 g of sodium acetate trihydrate and 1.0 g of sodium lignosulfonate were dissolved in 70 mL of ethylene glycol, and the mixture was continuously stirred until completely dissolved;

[0039] Step 2. Transfer the solution obtained in step 1 to a high-pressure reactor equipped with a polytetrafluoroethylene liner, put it into an oven that has been heated to 200 ° C, react for 12 hours, and then cool to room temperature with the furnace;

[0040] Step 3. Pour out the supernatant in the reaction kettle, take out the precipitate, wash with ethanol and deionized water at least three times, and dry at 60°C for 8 hours to obtain the sample Fe 3 o 4 -SL, using SEM to characterize it, it can be seen that it is a microsphere with a particle size of about 600nm, as shown in Figure 1 (A);

[0041] Step 4, under neutral environment, get the Fe that 0.02g step 3 obtains 3 o ...

Embodiment 2

[0044] Example 2, Fe 3 o 4 -Cu 2+ The detection steps of the simulated enzyme as a peroxidase to hydrogen peroxide are as follows:

[0045] Step (1), the Fe obtained in Example 1 3 o 4 -Cu 2+ The simulated enzyme is dispersed in water to produce 0.8mg / mL Fe 3 o 4 -Cu 2+ Simulated enzyme dispersion;

[0046] Step (2), get 50 μ L of Fe prepared in step (1) 3 o 4 -Cu 2+ The simulated enzyme stock solution was added to 1850 μL of 0.1M HAc-NaAc buffer solution (pH=3); then 50 μL of 32 mM 3,3',5,5'-tetramethylbenzidine salt was added to the reaction system Hydrogen peroxide (TMB) was used as the colorimetric reaction substrate; 50 μL of hydrogen peroxide solution with concentrations of 0.2mM, 0.3mM, 0.4mM, 0.6mM, 1mM, 2mM, and 3mM was added, and incubated at 45°C for 35 minutes to obtain a concentration of 5μM, 7.5μM, 10μM, 15μM, 25μM, 50μM, 75μM hydrogen peroxide solution;

[0047] Step (3), get the absorbance value of known hydrogen peroxide concentration solution tha...

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Abstract

The invention relates to the preparation and application of a complex metal chelate mimetic enzyme of ferric oxide. The present invention adopts hydrothermal method to synthesize Fe 3 o 4 ‑SL, SL molecules derive large RCOO‑groups, adsorbed on Fe 3 o 4 On the surface of the powder, the metal ions are covered by Fe under mild conditions 3 o 4 ‑SL is adsorbed to form nanocomposites, and the particles have good dispersion due to electrostatic and steric effects; and the prepared Fe 3 o 4 Composite metal chelates have good morphology and small particle size, have excellent peroxidase catalytic ability, and the separation process is simple, convenient, stable and reusable; based on Fe 3 o 4 The complex metal chelate mimics the excellent peroxidase activity of the enzyme, and a highly sensitive, low-cost, and simple method for the detection of hydrogen peroxide is established. The reaction condition is mild, the detection speed is fast, the sensitivity is high, and the selectivity is good, which can realize the visual and rapid identification and detection of hydrogen peroxide, and has high use value.

Description

technical field [0001] The invention belongs to the technical field of nanotechnology and analysis and detection, and in particular relates to the preparation and application of a ferric oxide complex metal chelate mimetic enzyme. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) is one of the most important reactive oxygen species, and has been widely used in many fields such as biology, chemistry, pharmacy, food engineering, and environmental testing, and it is also because of its excessive use in many fields, which directly or indirectly leads to many health problems. and environmental issues. Therefore, detecting H 2 o 2 Especially important, there are a variety of detection methods nowadays, such as spectrophotometry, electroanalysis, resonance light scattering analysis, chromatography, etc., but these methods have some disadvantages, such as high analysis cost, long analysis time, and analysis process. Complexity, low signal-to-noise ratio, low sensitivit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J31/28B01J37/10G01N21/78G01N21/31
CPCB01J31/28B01J37/10G01N21/31G01N21/78
Inventor 刘红英祝浪浪马焕樊凯李杜鹃
Owner HANGZHOU DIANZI UNIV
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