Solid Substrates for Mitigating or Preventing Cell and Tissue Adhesion and Vascularization
a technology of solid substrates and cells, applied in magnetic recording, calcium/strontium/barium carbonates, record information storage, etc., can solve the problems of severe vision loss or blindness, abnormal and fragile new blood vessels,
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example 1
Physical Properties Variability in Coralline-Based Solid Substrates of this Invention
Materials and Methods
[0267]A diamond disk saw was used to remove an outer coral layer, and large sections from which representative smaller sections of desired dimensions were cut from the coral block.
[0268]Coral from the hydrocoral Porites lutea which has an average pore size of 100-150 μm was harvested from various regions within a coral. The coral was evaluated visually for its appearance, density, and porosity. Coral was then optionally immersed in 5% sodium hypochlorite for removal of external organic tissue. Briefly, coral was first exposed to a 5% sodium hypochlorite solution for 30 minutes, 3 exchanges at temperature range RT at 50° C., and subatmospheric pressure using vacuum pressure ranging from 0.2-0.00001 Bar. The coral sections were then exposed to a 10% solution of hydrogen peroxide for 15 minutes at a temperature range of from RT-50° C., and subatmospheric pressure using vacuum press...
example 2
Structural Differences as a Function of Certain Physical Properties in Coralline-Based Solid Substrates of this Invention
[0276]In order to assess the consequence of the phenotypic variability in blood absorption in the plugs of Example 1, coral plugs were prepared using a standard production method including three hypochlorite washes, hydrogen peroxide treatment and multiple DDW washes. Their spontaneous fluid uptake and total fluid uptake values were determined. The implants were drilled and sterilized as was previously described.
[0277]Implants were processed for environmental scanning electron microscopy according to standard methods. FIG. 3A-C demonstrate the surface structure of a solid substrate with a higher specific fluid uptake capacity value as compared to that of substrates with a lower specific fluid uptake capacity value (compare FIGS. 3A-C and 3D-F). Substrates with a lower specific fluid uptake capacity value exhibited a smoother outer surface structure, as compared to...
example 3
Establishing a Screening Protocol for Coralline-Based Solid Substrates
[0278]Based on the findings in Examples 1 and 2, a screening protocol was established to select for an optimized coral-based solid substrate for mitigating or preventing cell or tissue adherence and / or vascularization. The coral sample being assessed will be dry, and in some aspects, it may be dried under vacuum and / or heated toward this end. A dry weight for each sample may be recorded. Fluid as described herein may be added to each assay container. In some embodiments, at least a 1:1 ratio or excess of the size of the sample in mm to the volume of fluid added in ml is applied to the container. The coral sample is then brought into contact with the fluid and the weight of the coral sample is assessed. A spontaneous fluid uptake value is established, based on either the complete uptake of the volume applied to the sample or based on the change in weight of the sample. Samples may optionally be dried, prior to furt...
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