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Ldl-cholesterol-lowering cell extract and food supplement

a technology of low-density lipoprotein and cell extract, which is applied in the direction of drug composition, bacteria material medical ingredients, metabolic disorders, etc., can solve the problems of liver damage, potential drug-drug interaction, and adverse effects of cholesterol-lowering therapeutic drugs,

Inactive Publication Date: 2021-09-09
EZCOL BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention relates to a petroleum ether extract of Rhodospirillum rubrum that can be used as a medicament and in the treatment of cardiovascular disease, atherosclerosis, dyslipidemia, arteriosclerosis, hypercholesterolemia, familial hypercholesterolemia, hyperlipidemia, inflammation, inflammatory disease, ischemia, infection, and a food supplement with LDL-cholesterol lowering properties. The petroleum ether extract is obtained by extraction of Rhodospirillum rubrum cells with a mixture of petroleum ether with a boiling point of between 60° C. and 80° C. and methanol containing about 0.03% by weight sodium chloride. The extract has a unique composition and can lower LDL-cholesterol in blood plasma. The method for producing the extract involves mixing the cells with the mixture and isolating it from methanol containing about 0.03% by weight sodium chloride.

Problems solved by technology

In addition, adverse effects associated with therapeutic drugs to control cholesterol levels, such as myopathy, liver damages and potential drug-drug interaction, have been reported.
None of these documents, however, provides information that a specific part of the cell and / or a specific fraction of the cell obtained upon subjecting the cells to a detailed extraction protocol, would be responsible for the alleged pharmaceutical activity.

Method used

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  • Ldl-cholesterol-lowering cell extract and food supplement
  • Ldl-cholesterol-lowering cell extract and food supplement

Examples

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examples

Experimental Procedures

[0134]The Rhodospirillum rubrum strain S1H was stored in liquid nitrogen in a 10% w / w sucrose-0.85% w / w saline solution. To regrow the strain, the cells were taken out the liquid nitrogen and thawed for 30 minutes at room temperature. Cells were streaked on a sistrom succinate agar plate and a rich Luria Bertani (LB) medium to grow colony forming units. The agar plates were incubated at 30° C. in dark and aerobic conditions for up to 4 days.

[0135]After 4 days, 10 single colonies were picked up and transferred to 10 tubes with 2 mL of Sistrom succinate liquid medium and incubated at 30° C. in dark, aerobic and orbital shaking at 150 rpm. After 4-5 days, the cells were grown and reached an OD680=0.5-0.6. To check the axenicity of the cultures, the cells were streaked on a Sistrom succinate agar plate and a rich LB medium agar plate and incubated up to 1 week to look for heterotrophic contaminants. When the axenicity check was approved, the 2 mL cultures were tra...

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Abstract

An extract of Rhodospirillum rubrum includes rhodovibrin, 1-hydroxyspirilloxanthin, 3,4-didehydrorhodopin, chloroxanthin, bacteriopheophytin a, rhodopin, spirilloxanthin, 3,4-dihydrospirilloxanthin, phytyl derivative of bacteriopheophytin a, ubiquinol-10, ubiquinone-9, ubiquinone-10 and rhodoquinone-10. Such an extract could be used as a medicament, for lowering of LDL-cholesterol in blood plasma of a subject and / or for subjects who suffer from or have an increased risk of one or more of cardiovascular disease, atherosclerosis, dyslipidemia, arteriosclerosis, hypercholesterolemia, familial hypercholesterolemia, hyperlipidemia, an LDL plasma level of at least 70 mg / dL, a total cholesterol level of at least 200 mg / dL, an Lp(a) level of at least 14 mg / dL, inflammation, inflammatory disease, ischemia, or infection.

Description

TECHNICAL FIELD OF THE INVENTION[0001]The present invention relates to a low-density lipoprotein (LDL)-cholesterol-lowering preparation, in particular to a bacterial cell extract for use as a medicament for lowering of the blood plasma LDL-cholesterol level. More specifically, the present invention relates to a petroleum ether extract of Rhodospirillum rubrum obtainable by extraction of Rhodospirillum rubrum cells with a mixture of petroleum ether with a boiling point of between 60° C. and 80° C. and methanol comprising sodium chloride. The invention also relates to the petroleum ether extract of Rhodospirillum rubrum of the invention, for use in a method for the lowering of LDL-cholesterol in blood plasma of a subject. The invention further relates to a pharmaceutical preparation, a food supplement containing said preparation, a foodstuff containing said food supplement, and to a method for their preparation.BACKGROUND OF THE INVENTION[0002]Cardiovascular disease is caused by a num...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/74A61P3/06A61K9/00A61K47/06
CPCA61K35/74A61K47/06A61K9/0056A61P3/06A23L33/13A61P1/16A23L33/135A23V2002/00A23V2200/3262A23V2200/326
Inventor WATTIEZ, RUDDYMASTROLEO, FELICESUTERS, ROBERTUS CHRISTIAAN JOSEPHUS
Owner EZCOL BV
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