The invention provides a 3D
mammary gland printing method based on HAMA fat
cell microspheres and
mammary gland organs, relates to the technical field of
biomedicine, and aims to solve the problems of incomplete structural function
simulation, low
cell survival rate, uneven distribution and the like in the prior art. According to the core technical scheme, HAMA fat
cell microspheres serve as fat layer function units,
mammary gland organs serve as gland layer units, and precise positioning of a double-layer structure is achieved through a double-
nozzle 3D printer; the
survival rate of adipose cells is improved by regulating and controlling the crosslinking degree of HAMA and the
porosity of the microspheres; special bio-ink and optimized printing parameters are adopted, so that the agglomeration rate of a functional unit is smaller than or equal to 5%, and a cross-linking
transition layer is arranged on an interface to promote structural connection; by adding
gelatin and adopting a dual-crosslinking process, the compression modulus of the model reaches 10-15kPa, the degradation period is 3-6 months, and the model has good mechanical properties and
biocompatibility. The application effectively simulates the physiological microenvironment of the mammary gland, and has important application value in the fields of
tissue engineering reconstruction,
drug screening,
disease research and the like.