The present application belongs to the technical field of micro-
plant landscape construction, and discloses a micro-
plant landscape in a
tissue culture bottle with an aesthetic appearance, a construction method and application, comprising the following steps: selection and disinfection of explants; explant
cutting; explant
inoculation; obtaining subculture seedlings after establishing primary sterile
tissue culture seedlings; and micro-
plant landscape design. The present application selects various plants with rich colors to match the micro-plant landscape, and uses plant colors and shapes to create the micro-plant landscape, thereby improving the ornamental value, broadening the product market, and promoting the rapid development of the micro-plant landscape industry.