Method and apparatus for detecting microbe by piezoelectric quartz crystal sensor

Abstract

A method and device for detecting microbe with piezoelectric quarts crystal transducer includes leading microbial metablin CO2 from culture cell to basic solution detection cell, measuring reaction variation with transducer and counting it out with frequency counter, obtaining linear relation between FDT and microbial concentration for carrying out microbial quantitative and qualitative detection by measuring response curve of microbe in different concentration.

Description

technical field [0001] The invention belongs to the field of microbial sensors, in particular to a method and device for detecting microorganisms by using a piezoelectric quartz crystal sensor. Background technique [0002] Traditional microbial determination methods include cell counting method, turbidimetric method, dry weight method, and hyphae length determination method. These methods have disadvantages such as cumbersome operation and long measurement cycle, and cannot meet the needs of rapid microbial determination in practical applications. In recent years, there have been many methods for measuring the thermal, optical, electrochemical and biochemical properties of microorganisms, such as non-biological electrochemical methods, bioelectrochemical methods, and cell component assays. [0003] Microbiological determination methods play an important role in many fields such as food inspection, clinical medicine and environmental monitoring. Tod...

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Problems solved by technology

High accuracy is one of its biggest advantages, but this method is time-consuming and cannot meet the requirements of rapid diagnosis

A biochemical method based on enzyme-linked immunosorbent assay has also been used for the detection of Mycobacterium tuberculosis. This method has high selectivity, high sensitivity, and low cost, but its operation is cumbersome and the possibility of false positives is high.

The application of DNA and RNA probes and polymerase chain amplification reactions will also cause a large number of false positive results, and the experimental equipment is expensive, which is difficult to promote in developing countries

The large-scale instruments for rapid detection of tuberculosis most commonly used in large-scale hospitals include the Bactec 460 system and the Bactec MGIT 960 system. The Bactec 460 system has disadvantages such as radioactive contamination, difficulty in instrument operation and maintenance, and expensive instruments.

Although the Bactec MGIT 960 system has basically realized automation, all reagents are dependent on imports, and the cost of the instrument itself is high, so its promotion is limited by economic conditions, and it is within reach in poor countries or developing countries with a high incidence of tuberculosis. Inaccessible, and the detection time is relatively long. Generally, it takes about 8-10 days for a positive result to come out quickly, about a month for a slow one, and about 40 days for a negative result.

In our research group, a piezoelectric immunosensor based on PQC mass response has been designed. It is simple and easy to operate, but it is not sensitive enough, and the antibody is expensive and difficult to obtain; another FDT piezoelectric bulk acoustic impedance sensor has also been developed. It is used to detect Mycobacterium tuberculosis with fast response and high sensitivity, but it is necessary to control the conductance of the medium to make it work within the sensitivity range of the instrument, which often results in the inhibition of bacterial growth or no growth at all due to lack of nutrients

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