Chymosin powder preparation method
A technology of rennet and aluminum oxide, applied in the direction of hydrolytic enzymes, etc., can solve the problems affecting the quality of cheese products, turbidity of enzyme liquid, unpleasant odor, etc., and achieve the advantages of simple and easy extraction method, high enzyme activity and avoiding pollution Effect
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Embodiment 1
[0012] The method has the following sequential steps:
[0013] a. Use the sucking calf abomasum as raw material, remove the contents, peel off the fat and connective tissue on the surface of the abomasum, and wash it clean. Grind 1000g of calf abomasum with an electric meat grinder, add 5000ml of water, add salt with a concentration of 1mol / 1, stir and soak at room temperature for 72 hours, then centrifuge at 4000r / min for 15min to remove the residue, and obtain enzyme-containing The supernatant was concentrated by ultrafiltration and vacuum freeze-dried to obtain preliminary crude rennet powder.
[0014] b. Add 500ml of water to the coarse rennet powder to make it into a concentrated enzyme-containing aqueous solution, and then add 2g of gamma-alumina per 100ml of it (gamma-alumina has a surface area of 150-200m 2 / g, the average pore diameter is 150-200 Ȧ), it is carried out the adsorption of chymosin, is stirred, stands still for 5 hours, then centrifuges to remove the s...
Embodiment 2
[0018] The method has the following sequential steps:
[0019] a. Use the sucking calf abomasum as raw material, remove the contents, peel off the fat and connective tissue on the surface of the abomasum, and wash it clean. Grind 1000 g of calf abomasum with an electric meat grinder, then add 7000 ml of an aqueous solution with a concentration of 2 mol / l sodium chloride, stir and soak at room temperature for 48 hours.
[0020] b. Then add 3g of γ-alumina to every 100ml of it (the surface area of γ-alumina is 150-200m 2 / g, the average pore diameter is 150-200 Ȧ), carry out the adsorption of chymosin, stir, let stand for 5 hours, centrifuge again and remove the supernatant, obtain γ-alumina precipitate.
[0021] c. Use 600 ml of an aqueous solution with a pH of 5.3 to elute and analyze the γ-alumina precipitate, and then centrifuge to remove the γ-alumina residue to obtain 500 ml of an enzyme-containing solution.
[0022] d. Concentrate 500ml of the enzyme-containing soluti...
Embodiment 3
[0024] The method steps of this embodiment are the same as in Example 1, except that the soaking time is 60 hours, and its concentration is to add 4g of gamma-alumina (the surface area of gamma-alumina is 150-200m2) in every 100ml. 2 / g, the average pore diameter is 150-200 Ȧ).
[0025] Finally, the refined rennet powder is obtained by vacuum freeze-drying, which is a white or light yellow solid powder with a slight salty taste and hygroscopicity, no peculiar smell, and the enzyme activity is stable in a dry state. It is unstable in aqueous solution, and it will be inactive when the temperature is below 15°C or above 55°C, and the optimum temperature is 37-43°C.
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