Portable fast separation device of pine material nematode and its separation method

Abstract

The invention relates to a portable pine nematode fast-separating device and method, belonging to forestry technical field. The device comprises water saver, sample vessel, water pump, testing sample, water inlet pipe, hole stopper, and fastening tape. And the method uses the water pump to pump the water in the water saver and presses the water into the testing sample, extrudes the high pressure water from the testing sample to flow through the sample vessel back to the water saver, repeats in this way for 6 min to complete the separation; then places separated solution in a culture vessel, and observes and identifies the separated solution under a microscope. The invention is convenient to carry and operate and quick to sample, has good sampling effect and can be applied to detect logs and sheets, and to fast separate nematodes of various trees either.

Description

technology field; [0001] The invention relates to a portable rapid separation device for pine xylophilus and a separation method thereof, belonging to the field of forestry detection devices and detection technologies. Background technique: [0002] There are many nematodes on pine trees, among which pine wilt disease caused by pine wood nematode (Bursaphelenchus xylophilus) is a very dangerous pine tree disease, which can cause a large number of pine trees to die within a few months, and is listed as an external and internal quarantine object. Because of its serious harm, rapid spread, and extremely difficult to control, it is called "forest fire without smoke" and "cancer of pine trees". [0003] Quickly separate nematodes from pine wood and carry out rapid and accurate identification to determine whether there is pine wilt nematode. Very important basic work and key technologies in diagnosis and other aspects: it is one of the important contents of the forestry departmen...

AI Technical Summary

Problems solved by technology

But there are still some problems to be studied and solved: (1) the device uses a vacuum pump to pump air, which requires a good seal, and the technical requirements are difficult, and it is not easy to seal; The packing box is made of wooden boards. The wooden boards are cut and sawn along the longitudinal axis of the logs. The conduction tissue and turpentine channel tissue on the wooden boards are along the direction of the board surface instead of perpendicular to the board surface. Use this device to detect pine boards. It is not feasible to allow water to filter out nematodes along the pine wood conduction tissue and rosin channel tissue; it cannot be detected with this device for laminates, and the scope of application is limited; (3) a vacuum pump is used in this device, which is large in size and heavy in weight

The suction filter cartridge and the sleeve are embedded into the pine wood to be inspected by grinding the blade-like edge, and need to be equipped with pressurized equipment, which increases the volume and weight, making the whole device large in size, heavy in weight, inconvenient to carry, and expensive. high

[0007] Through literature search, do not find the public report identical with the technical scheme of the present invention

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