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Bifidobacteria exocellular polysaccharide and its production method and special purpose production strain

A technology of bifidobacteria and extracellular polysaccharide, applied in bacteria, fermentation and other directions, can solve the problem of few researches on bifidobacteria metabolites, and achieve the effects of low equipment requirements, simple operation and simple raw materials

Inactive Publication Date: 2008-02-13
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on bifidobacterial metabolites

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1, Isolation and Identification of Bifidobacterium asteroides BM-10CGMCC №1355

[0027] 1. Screening of bacterial strains

[0028] Intestinal samples of long-lived elderly people in Bama, Guangxi, collected through sterile bags, were promptly transferred to the ultra-clean workbench, weighed 25 grams with sterile paper, dissolved in 225 mL of normal saline, and oscillated evenly to obtain 10 -1 bacterial suspension. Take 1mL of this bacterial suspension with a needle tube and inject it into a 9mL normal saline tube to obtain a dilution of 10 -2 The bacterial suspension was diluted stepwise under anaerobic conditions until 10 -10 .

[0029] Take 0.4mL of each dilution respectively, inoculate to add 0.3% sterile CaCO 3 Powdered modified MRS medium (peptone 1%, beef extract 1%, yeast extract 0.5%, dipotassium hydrogen phosphate 0.2%, ammonium citrate 0.2%, sodium acetate 0.5%, glucose 2%, MgSO 4 .7H 2 O 0.058%, MnSO 4 .7H 2O 0.025%, Tween 800.1%, cysteine ​...

Embodiment 2

[0038] Example 2, Utilizing Bifidobacterium asteroides BM-10CGMCC No. 1355 to produce extracellular polysaccharide

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PUM

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Abstract

The invention provides a polysaccharide outside the cells of bifidobacteria and its making, and preparing-purpose strain. Polysaccharide outside the cells of bifidobacteria offered in said invention is prepared by fermenting star-shaped BM-10 CGMCC No 1355 (Bifidobacterium ast eroides). BM-10 CGMCC No 1355(star-shaped Bifidobacterium asteroides) comes from the intestinal canal of the aged in Bama of Guangxi province with reliable safty, could be made into live bacteriostatic and delete the processes of common fermentation, extraction and so on. After the optimization to the synthetic condition of EPS produced by BM-10 CGMCC No 1355(star-shaped Bifidobacterium asteroids), its product could reach 1.21g / L. The method and raw material of preparing polysaccharide outside the cells of bifidobacteria in said invention is simple and suitable for industrial production with low requirements to devices, simple operation, low cost.

Description

technical field [0001] The invention relates to a bifidobacterium extracellular polysaccharide, a production method thereof and a special production strain. Background technique [0002] Bifidobacterium (Bifodobacterium) mainly exists in the intestinal tract of humans and animals, and exerts physiological effects on the host such as biological barrier, nutrition, immunity, control of endotoxemia, anti-aging, and anti-tumor. It is a typical beneficial bacterium in the human intestinal tract, and its growth and reproduction run through the entire life course of a person. Bifidobacteria grow and reproduce rapidly in an anaerobic environment to produce a large amount of lactic acid, lower the pH value of the system to inhibit and kill intestinal pathogenic bacteria, and maintain a normal micro-ecological balance of the flora. The presence and amount of bifidobacteria is a sign of human health. If the growth of bifidobacteria in the body stops, it will be difficult for humans a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C12P1/04C08B37/00
Inventor 李平兰欧阳清波李伟欣张篪郑海涛马长伟
Owner CHINA AGRI UNIV