Unlock instant, AI-driven research and patent intelligence for your innovation.

Miniature enzyme reactor with controllable size and property and its preparation method

A kind of technology of enzyme reactor and nature, which is applied in the field of miniature enzyme reactor and its preparation, can solve the problems of unsuitable high molecular weight substrate, restriction of wide application, diffusion limitation, etc., to achieve performance optimization, facilitate substrate diffusion, and enzyme activity Improved effect

Inactive Publication Date: 2008-04-23
BEIJING UNIV OF CHEM TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The covalent coupling method shows good stability, but the covalent coupling reaction is easy to denature and inactivate the enzyme; the cross-linking method is more likely to inactivate the enzyme; Suitable for mass transfer of high molecular weight substrates and column reaction systems, and often have problems such as diffusion limitations
The inadequacies of the above-mentioned methods for immobilizing enzymes limit their wide application. Therefore, the research and development of simple and practical immobilization methods and novel enzyme immobilization materials with excellent design, synthesis and application performance are the most important methods for immobilization at present. One of the focus of enzyme research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Miniature enzyme reactor with controllable size and property and its preparation method
  • Miniature enzyme reactor with controllable size and property and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: A. adopt hydrothermal synthesis method to prepare nanoporous material SBA-15 (aperture is 6.6nm), take polyethylene glycol-polypropylene glycol-polyethylene glycol triblock copolymer (P123) as template agent, Using tetraethyl orthosilicate (TEOS) as the silicon source, the synthesis process is as follows: at 45°C, dissolve 2g of P123 in a mixed solution of 10ml of HCl and 60ml of deionized water, stir for 3h, then add 4.27g of TEOS and stir for 24h, The solution was transferred to a hydrothermal synthesis reactor and placed in an oven at 100°C for 48h. Then suction filtered and washed with deionized water. Using a Soxhlet extractor, first use ethanol as a solvent for reflux extraction for 8 hours, then use tetrahydrofuran as a solvent for reflux extraction for 8 hours, and then use carbon tetrachloride for reflux extraction for 8 hours. Reflux extraction with Soxhlet extractor 3d) Finally, wash with ice acetone for 3 times and dry to obtain the carrier of...

Embodiment 2

[0027] Example 2: A. The nanoporous material SBA-15 (pore size: 6.6nm) was prepared by hydrothermal synthesis, calcined in a muffle furnace at 400° C. for 6 hours, and cooled to obtain the carrier of sample 2.

[0028] Subsequent steps are the same as in Example 1, only this sample (sample 2) is substituted for sample 1, and the results are shown in Table 1.

Embodiment 3

[0029] Example 3: A. Nanoporous material SBA-15 (pore size: 6.6nm) was prepared by hydrothermal synthesis, calcined in a muffle furnace at 400°C for 12 hours, and cooled to obtain the carrier of sample 3.

[0030] Subsequent steps were the same as in Example 1, only this sample (sample 3) was substituted for sample 1, and the results are shown in Table 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to View More

Abstract

The present invention provides one kind of miniature enzyme reactor with controllable size and property and its preparation, and belongs to the field of immobilized enzyme technology. The miniature enzyme reactor has 1D or 3D through hole structure with enzyme molecule fixed inside the holes physically or chemically, and the features of controllable size, surface functional radical types and number and fixed enzyme amount. By means of regulating the type and amount of functional radicals on the surface of the nanometer porous material and fixing enzyme, the miniature enzyme reactor with controllable size and property is constructed. The present invention has the advantages of regulation of enzyme fixing amount and the optimization of the immobilized enzyme property. The present invention makes it possible to realize the development, application and industrial production of molecular level enzyme / carrier design and control.

Description

technical field [0001] The invention belongs to immobilized enzyme technology, in particular, it provides a miniature enzyme reactor with controllable size and properties and a preparation method thereof. Background technique [0002] As an interdisciplinary technology, immobilized enzyme technology has been widely used in the fields of life science, biomedicine, food science, chemical engineering and environmental science. Immobilized enzyme technology is a technology developed based on the shortcomings of free enzymes, such as instability, easy loss, difficulty in separating from substrates, difficulty in reutilization, and high cost of single use. The above shortcomings of enzymes, and constantly seek more perfect immobilization methods and better immobilization carriers. At present, enzyme immobilization methods can be divided into four categories: adsorption method, covalent coupling method, cross-linking method, and embedding method. Although the adsorption method ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/00C12N11/02
Inventor 何静刘治军徐燕段雪
Owner BEIJING UNIV OF CHEM TECH