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Preparation method of magnetic nanometer granule containing mitocin-C

A technology of magnetic nanoparticles and mitomycin, which can be used in medical preparations containing active ingredients, organic active ingredients, pharmaceutical formulations, etc. Beneficial for parenteral administration, improved bioacceptability, and small particle size

Inactive Publication Date: 2008-04-30
SHANGHAI INST OF PHARMA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the method for preparing drug-containing magnetic nanoparticles, such as the preparation and characteristic research of doxorubicin magnetic gelatin microspheres (Wu Chuanbin He Sumei, Chinese Journal of Pharmaceutical Industry. 1994.25 (2). 63-66), is in the preparation of During the process, a cross-linking agent is added, but this method introduces other chemical components, which is not conducive to the treatment of diseases. At the same time, due to the reaction between the cross-linking agent and the active ingredient, it may affect the efficacy and safety of the active ingredient.

Method used

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  • Preparation method of magnetic nanometer granule containing mitocin-C
  • Preparation method of magnetic nanometer granule containing mitocin-C
  • Preparation method of magnetic nanometer granule containing mitocin-C

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Dissolve 150 mg of albumin in 0.5 ml of Fe with a weight concentration of 105 mg / ml 3 o 4 A magnetic fluid formed with water, and then 0.75ml of mitomycin C with a weight concentration of 13.3mg / ml was mixed to obtain an aqueous phase;

[0035] (2) Using an emulsifier for the water phase and the oil phase, stirring and emulsifying at a temperature of 25° C. to obtain an emulsion;

[0036] Said emulsifier is the mixture of lecithin and cholesterol, and its weight ratio is:

[0037] Lecithin: cholesterol = 4:1;

[0038] Said oil phase is refined soybean oil, wherein the surfactant is lecithin: cholesterol=4:1; the weight content is 1.5% of the refined soybean oil weight;

[0039] The weight ratio of water phase and oil phase is: water phase: oil phase=1: 350;

[0040] The stirring speed is 10000rpm, and the stirring time is 10 minutes;

[0041] (3) heating the emulsion at 80° C. for 10 minutes to solidify, then cooling to room temperature, centrifuging, washing wit...

Embodiment 2

[0048] (1) Dissolve 220mg of albumin in 0.5ml of Fe with a weight concentration of 210mg / ml 3 o 4 A magnetic fluid formed with water, and then 0.75ml of mitomycin C with a weight concentration of 26.6mg / ml was mixed to obtain an aqueous phase;

[0049] (2) Using an emulsifier for the water phase and the oil phase, stirring and emulsifying at a temperature of 25° C. to obtain an emulsion;

[0050] Said emulsifier is Span 80,

[0051] Said oil phase is refined soybean oil, wherein the surfactant is Span 80, and the weight content is 1.5% of the weight of refined soybean oil;

[0052] The weight ratio of water phase and oil phase is: water phase: oil phase=1: 550;

[0053] The stirring speed is 3000rpm, and the stirring time is 20 minutes;

[0054] (3) heating the emulsion at 100° C. for 60 minutes to solidify, then cooling to room temperature, centrifuging, washing with petroleum ether, and drying to obtain magnetic nanoparticles containing mitomycin C;

[0055] Components ...

Embodiment 3

[0061] An important evaluation index of targeted preparations is the targeting of the preparation to the target site. After the administration of targeted preparations, the drug should be mainly concentrated in the target site, and the blood is only used as a place for drug transport and exchange, and its drug concentration should be far lower than the drug concentration in the target area. In this sense, the hemodynamic changes of the drug have no It cannot fully reflect the action process of the drug in the body. Nevertheless, due to the organic connection between the target organ and the blood, there must be a certain relationship between the drug concentration in the target organ and the blood drug concentration.

[0062] Therefore this part takes the MMC-MNP (without adding magnetic field) of embodiment 1 and two groups of MMC solutions as contrast, utilizes high-performance liquid chromatography to measure the content of medicine, has studied MMC-MNP (adding magnetic fie...

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Abstract

The invention provides a process for preparing magnetic nano particles containing mitomycin, which consists of the following steps: (1) dissolving aqueous solution of albumin into magnetic liquid formed by Fe3O4 and water, mixing with mitomycin C to obtain aqueous phase, (2) emulsifying the aqueous phase and oily phase with emulsifying agent, (3) heating the emulsion for 5-60 min at 60-100 deg C for solidification, then collecting the particles through conventional methods. The invention can realize the advantages of {>better dispersion in the system, small grain size and large specific surface area.

Description

technical field [0001] The invention relates to a preparation method of a mitomycin C preparation, in particular to a preparation method of magnetic nanoparticles loaded with mitomycin C. Background technique [0002] Cancer is a large class of diseases that seriously endanger human health. At present, in addition to surgical resection and radiotherapy, chemotherapy is still an important means of cancer treatment. [0003] Mitomycin C (MMC) is a broad-spectrum anticancer drug of antibiotics. It was discovered by Hata et al. in 1955. It has been used clinically for more than 20 years and is one of the effective drugs for treating various cancers. There is a dosage form of powder injection, which is mainly used clinically for digestive tract cancers such as gastric cancer, intestinal cancer, liver cancer, esophageal cancer, pancreatic cancer, etc. It is also effective for lung cancer, breast cancer, cervical cancer, etc. In addition, it can also be used for malignant Lymphom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/407A61K9/14A61K47/02A61K47/42A61P35/00
Inventor 陆伟根朱瀛张军
Owner SHANGHAI INST OF PHARMA IND CO LTD