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Determination of protein content in thermosetting polymer

A protein content, polymer technology, applied in the measurement of color/spectral properties, material analysis by observing the effect of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of inability to measure cross-linked polymers. Protein content and other problems in the medium, to achieve the effect of simple measurement method and solve the problem of determination

Inactive Publication Date: 2008-08-20
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the non-radioactive isotope method cannot be used to simply measure the protein content in cross-linked polymers in the existing protein content determination technology, and to provide a method for measuring the protein content in thermosetting polymers

Method used

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Examples

Experimental program
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Embodiment 1

[0027] - Drawing of standard curve

[0028] In 5 test tubes, add 5 mL of casein aqueous solution with mass concentration of 1.0, 2.0, 3.0, 4.0 and 5.0 mg / mL respectively, and slowly add 2 mL of NaOH solution with mass concentration of 0.1 g / mL into each test tube , after shaking well, add dropwise 1mL of CuSO with a mass concentration of 0.01g / mL 4 solution, and keep shaking the system, so that Cu 2+ Fully complex with casein until flocculent precipitation appears, let it stand for 15 minutes, filter, and dilute the filtrate 25 times. Measure the absorptivity with atomic absorption spectrometer respectively, make the standard curve of absorbance A and casein concentration relation;

[0029] - Labeling of proteins in polymers

[0030] Within the temperature range of 25°C, 5 mg of casein-containing polymer was suspended in 10-20 mL of aqueous solution, and 2 mL of NaOH solution with a mass concentration of 0.1 g / mL was slowly added dropwise. After shaking well, add dropwise ...

Embodiment 2

[0038]In 5 test tubes, add 7 mL of bovine serum albumin aqueous solution with mass concentration of 1.0, 2.0, 3.0, 4.0 and 5.0 mg / mL respectively, and slowly add 3 mL of bovine serum albumin solution with mass concentration of 0.1 g / mL into each test tube NaOH solution, shake well, add dropwise 1mL of CuSO with a mass concentration of 0.01g / mL 4 solution, and keep shaking the system, so that Cu 2+ Fully complex with bovine serum albumin until flocculent precipitation appears, let it stand for 20 minutes, filter, and dilute the filtrate 25 times. Measure the absorptivity with atomic absorption spectrometer respectively, make the standard curve of absorbance A and bovine serum albumin concentration relation;

[0039] - Labeling of proteins in polymers

[0040] Within the temperature range of 30°C, 7 mg of polymer containing bovine serum albumin was suspended in 10-20 mL of aqueous solution, and 4 mL of NaOH solution with a mass concentration of 0.1 g / mL was slowly added dropwi...

Embodiment 3

[0048] In 5 test tubes, add 10 mL of egg white lysozyme aqueous solution with mass concentration of 1.0, 2.0, 3.0, 4.0 and 5.0 mg / mL respectively, and then slowly add 4 mL of lysozyme with mass concentration of 0.1 g / mL into each test tube NaOH solution, shake well, add dropwise 2mL of CuSO with a mass concentration of 0.01g / mL 4 solution, and keep shaking the system, so that Cu 2+ Fully complex with egg white lysozyme until flocculent precipitation appears, let it stand for 30 minutes, filter, and dilute the filtrate 50 times. Measure the absorptivity with the atomic absorption spectrometer respectively, and make the standard curve of the relationship between the absorbance A and the concentration of egg white lysozyme;

[0049] - Labeling of proteins in polymers

[0050] In the temperature range of 50°C, 10 mg of polymer containing egg white lysozyme was suspended in 10-20 mL of aqueous solution, and 6 mL of NaOH solution with a mass concentration of 0.1 g / mL was slowly ad...

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Abstract

A method for determining protein content in heat - set polymer includes combining biuret reaction and high temperature ashing with atomic absorption spectrum to complex protein in polymer with copper first, then applying high temperature to decompose polymer, finally using atomic absorption spectrum to measure copper concentration and furthermore obtaining protein content.

Description

【Technical field】: [0001] The invention is used for the determination of protein content in thermosetting polymers. It is a development of the protein solution content determination method in analytical chemistry, and it can be an effective and simple method to determine the protein content in solids that are difficult to dissolve. 【Background technique】: [0002] Determination of protein content is an essential means in protein research. Generally, the physical properties of protein, such as refractive index, specific gravity, and ultraviolet absorption, can be used for preliminary determination. Then chemical methods were used for rigorous quantitative analysis. Previous chemical analysis methods, such as nitrogen determination and biuret method (see Levin, R.; Brauer, R.W. The biuret reaction for the determination of proteins: Animproved reagent and its application. J.Lab.Clin.Med., 1951 , 38, 474), Folin-phenol reagent method (seeing Lowry, O.H.; Rosebrough, N.J.; Far...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N21/82
Inventor 黄积涛王艳芬郑嗣华张嘉琪
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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