Microarray chip detection system

Abstract

This invention dicloses a microarray chip examination system composes, the embody incident light production system, the reflected light gathering system, has received the spotlight processing system as well as lays aside the microarray chip carrying table, the light production system includs a laser, a scattering beam collimation mirror as well as a cylinder shaper; the reflected light gathering system includs a pair of focal plane image formation set of lenses, a filter piece as well as a CCD detector. compares with the international popular biological chip instrumentation, this invention combines a scan and a digital imaging in a body, its structure simple, the cost will be inexpensive, will reduce in particular to the machine-finishing precision request, causes this invention in and the promotion and the popularization.

Description

technical field [0001] The invention relates to a microarray chip detection system, in particular to a microarray chip detection system with low cost and relatively high detection sensitivity and resolution. Background technique [0002] Biochip technology is a multidisciplinary interdisciplinary technology that emerged in the late 20th century. With the help of engineering technologies such as microelectronics, micromechanics, and optical-mechanical-electrical-software integration technology, it integrates sample preparation, chemical reactions, and analysis and detection in life science research. The discontinuous process is integrated into a small chip to achieve continuous, integrated and miniaturized processing. [0003] The biochip detection instrument is an inseparable special instrument and equipment for the practical application of biochip technology. With the help of microscopic magnification imaging, confocal scanning, fluorescence detection, and digital image pro...

AI Technical Summary

Problems solved by technology

The main disadvantages of confocal scanners are: 1. The detection resolution is determined by the imaging resolution of the optical system, the mechanical precision of the scanning motion platform, and the scanning motion control accuracy. In the application of scanning detection, it is difficult to greatly improve after reaching 5 microns; 2. The confocal scanner is a serial working mode, and the data acquisition and transmission speed is affected by scanning motion, A / D conversion and data interface communication, etc. Many limitations, large area (20mm × 60mm) detection time is relatively long (20 minutes / 5 micron resolution), it is difficult to greatly increase its speed; 3, the confocal scanner needs to increase the grating ruler in the actual structure As a feedback component to ensure scanning accuracy, the cost will increase by about 1 / 8; 4. The overall accuracy of the confocal scanner depends on the mechanical accuracy of the scanning motion platform, and the cost of machining is high

These factors make the cost of confocal scanners very expensive, and the general price is around 50,000 to 100,000 US dollars.

The refrigerated CCD imaging system is also a biochip detection instrument, which can perform large-area (tens of millimeters), low-resolution (>50 microns) biochip hybridization signal detection, and store and generate digital images. The main disadvantage of this system is Because: 1. The cost of the refrigerated CCD itself is relatively high, generally about 5,000 US dollars to 20,000 US dollars; 2. After using the refrigerated CCD, in order to improve the detection sensitivity, the sampling exposure integration time is usually extended (it takes tens of seconds or several minutes) This method will greatly increase the detection time, and will also accelerate the photobleaching of the tested samples in the biochip, which will greatly reduce the service life of the chip; The resolution of large-area chip detection is severely limited, otherwise the detection area will inevitably decrease; 4. If you choose a CCD chip with a large detection area, its cost will increase exponentially

[0005] These above-mentioned instruments still have such or such shortcomings, and their market prices are also very expensive, and are not suitable for popularization and application.

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