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Biological preparation method of agricultural amino acid and fertilizer product thereof

An amino acid and microbial technology, applied in the fields of organic fertilizer, organic fertilizer preparation, biological organic part treatment, etc., can solve problems such as threats to tobacco production and sustainable development of tobacco leaves, sharp decrease in soil microbial activity, and poor control of decomposing degree. Achieve the effect of low product price, stimulating plant growth and less investment in fixed assets

Active Publication Date: 2009-02-11
黑龙江大禾农业有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term application of inorganic fertilizers, coupled with the fact that almost all the aboveground parts of tobacco are harvested and taken away, will cause soil compaction, unbalanced nutrients, and a sharp decrease in soil microbial activity in the tobacco field, which will further lead to the occurrence of tobacco continuous cropping obstacles, seriously threatening tobacco production and production. Sustainable Development of Tobacco Leaf
Many tobacco farmers have realized the bad results caused by not using organic fertilizers on the tobacco soil for a long time. They often do some compost or retting on the field, and then apply it to the soil. However, due to poor control of the maturity of the tobacco, the top leaves of the tobacco are still significantly reduced.

Method used

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  • Biological preparation method of agricultural amino acid and fertilizer product thereof
  • Biological preparation method of agricultural amino acid and fertilizer product thereof

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Experimental program
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Effect test

Embodiment Construction

[0017] 1. Expanded culture of liquid strains

[0018] Preserved strains Slant activation (NB medium, cultured at 37 degrees for 24-48 hours) Bottle seed culture (seed medium, cultured at 37 degrees for 24-36 hours, rotating speed 120-200 rpm) Fermented seed culture (seed medium, 37-degree seed tank culture for 24-36 hours) Fermentation culture (liquid fermentation production formula, 37 degree fermenter for 24-36 hours fermentation broth Solid matter adsorption to form solid fermentation strains Store at low temperature (4-10 degrees) for later use.

[0019] The formula of the seed culture medium is: 0.1-0.5% of beef extract, 0.25-0.8% of peptone, 0.15-0.7% of glucose, 0.15-0.6% of sodium chloride, and pH7.0-7.5.

[0020] The formula of the liquid fermentation production medium is: 1.25-2.0% of corn flour, 1.8-3.0% of cake, 0.2-0.75% of sodium chloride, 0.01-0.1% of sodium carbonate, and 0.1%-0.2% of amylase.

[0021] 2. Preparation of solid fermentation material...

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Abstract

The invention discloses a preparing method and fertilizer product of agricultural amino acid, which is characterized by the following: separating microbe bacteria 37-1 to produce secretory proteinase from wild pig gastrointestinal; seeding the bacteria in the waste protein; emzymlyzing solid for 5d (30-50 deg.c); making free amino acid content at 12% as raw material of fertilizer.

Description

1. Technical field [0001] The invention relates to a biological production method of agricultural amino acids and the fertilizer product produced therefrom, which belongs to the intensive agricultural production technology and is specially used for increasing crop yield, improving crop quality, improving soil biological activity and the like. 2. Technical background [0002] Since the early 1970s, the large-scale application of chemical fertilizers has doubled the unit yield and total output of our country. In the past 25 years, due to the contribution of chemical fertilizers, China with a population of 1.3 billion has completely solved the problem of food security. It can be seen that the contribution of chemical fertilizers to crop production in my country and the whole world is indelible. [0003] However, just because of the contribution of chemical fertilizers in agricultural production, people now rely too much on chemical fertilizers, especially chemical nitrogen fert...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P13/04C05F17/00
CPCY02W30/40
Inventor 沈其荣杨兴明黄启为徐阳春
Owner 黑龙江大禾农业有限公司