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Low temperature protectant solution program controlled refrigerating mechanism for vitrified preservation

A technology of cryoprotectant and cooling device, which is applied in household refrigeration devices, preservation and application of human or animal bodies, etc., can solve the problems affecting the uniform diffusion of cryoprotectant, freezing of solution, etc., and achieve uniform concentration and temperature. Uniform, guaranteed uniformity effect

Inactive Publication Date: 2009-02-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To reduce toxic effects, biological materials need to be treated with cryoprotectant solutions at lower temperatures, but too low a temperature can also cause the solution to freeze, affecting the uniform diffusion of the cryoprotectant

Method used

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  • Low temperature protectant solution program controlled refrigerating mechanism for vitrified preservation
  • Low temperature protectant solution program controlled refrigerating mechanism for vitrified preservation

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Embodiment

[0019] Dimethyl sulfoxide is a common cryoprotectant, and the minimum concentration of the glass transition under normal pressure is 49% (mass percentage, the same below). Through the device provided by the invention, the concentration of the dimethyl sulfoxide solution can be increased from 10% to 49% within 20 minutes, and at the same time, the temperature of the solution can be kept 8° C. higher than the freezing point. The implementation steps are: 1) before the device is opened, put 20 grams of 10% dimethyl sulfoxide solution in the stainless steel container, and put 20 grams of pure dimethyl sulfoxide in the storage tank; 2) turn on the refrigeration device and electric heating to make the solution The temperature drops to 8°C above the freezing point temperature (-4.3°C) corresponding to the 10% dimethyl sulfoxide solution, which is 3.7°C, record this time as t 0 ; 3) at time t 0 Turn on the constant flow pump and the micro solenoid valve, so that the constant flow pum...

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PUM

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Abstract

The invention discloses a program-controlled cooling device of cryogenic protective agent solution for vitrification storage, comprising a pure cryogenic protective agent storing cistern, a constant flow pump, a stainless steel container for low-temperature protective agent, an electromagnetic stirring device, a refrigerating unit, a heating unit and a programmable controller. The invention is characterized in that pure cryogenic protective agent is continuously filled in the cryogenic protective agent storing cistern through the constant pump, temperature of the cryogenic protective agent is continuously decreased under the action of cooling medium provided by the refrigerating unit, the programmable controller controls the heating capacity of the heating unit according to the temperature signal detected by a platinum resistance, therefore, the temperature of the cryogenic protective agent solution is constantly higher than freezing point of 5 to 10 DEG C. The invention can be applied to low temperature storage of biological materials.

Description

technical field [0001] The invention relates to a program-controlled cooling device for cryoprotectant solution used for vitrification preservation. Background technique [0002] Cryopreservation of biological materials has been widely used in clinical medicine and biomedical research. Biomaterials can remain active for several months or decades at -80°C or -196°C. In order to avoid damage to biological materials during the process from room temperature to low temperature and from low temperature to room temperature, people use cryoprotectants (such as glycerol, dimethyl sulfoxide) to provide protection. The biological material is treated with a cryoprotectant solution before cooling from room temperature to low temperature, and the cryoprotectant is eluted after returning from low temperature to room temperature. The use of high concentrations of cryoprotectants can avoid the formation of ice crystals during freezing and various hazards caused by ice crystal formation. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02F25D9/00F25D29/00
Inventor 张绍志陈光明虞效益
Owner ZHEJIANG UNIV
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