The invention discloses disinfecting equipment and disinfection method of a biochemistry laboratory. The disinfection method comprises the following steps of a disinfection step: performing atomizingon disinfecting liquid by equipment, jetting and spouting atomizing granules from a jet nozzle to a laboratory through a fan, and at the time, sucking the air to the inner part of the equipment from aspraying gas inlet channel; and a filtration step: when an atomizing device does not work, sucking laboratory air from a filtration channel by the fan, performing filtering and performing spouting outwards. A liquid storing tank, an atomizing device, a first fan and the jet nozzle, which are in series connection, are arranged in the disinfecting equipment. In the disinfection step, air flows through the spraying gas inlet channel, and in the filtration step, air flows through the filtration channel. According to the disinfection method disclosed by the invention, through the two steps, rime fog in the laboratory environment can be thoroughly removed, substances in the air can be adsorbed and recovered, pollutants which are treated by the disinfecting liquid and a part of pollutants whichare not treated by the disinfecting liquid are removed, precision equipment is protected from being damaged, the disinfecting equipment is safe and harmless to a human body, laboratory pollution can be quickly and effectively removed, and the polluted laboratory can recover the clean state as soon as possible.