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Molecular detection of functional bacterium QX-5 for treating organic industrial effluent

A technology of industrial wastewater and functional bacteria, applied in the field of molecular biology, can solve the problems of heavy workload, rapid and accurate monitoring of functional strains of difficult microorganisms, and cumbersome steps

Inactive Publication Date: 2009-03-18
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current method of detecting its content in the reaction system is to use the traditional pure culture technology. This method has cumbersome steps and a large workload, and it is difficult to apply to the rapid and accurate monitoring of microbial functional strains in actual wastewater treatment projects.

Method used

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  • Molecular detection of functional bacterium QX-5 for treating organic industrial effluent
  • Molecular detection of functional bacterium QX-5 for treating organic industrial effluent
  • Molecular detection of functional bacterium QX-5 for treating organic industrial effluent

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Embodiment Construction

[0050] 1. Materials: The functional strain QX-5 that degrades a variety of organic acids, sugars, and alcohols, Brevibacterium, has been used in the treatment of brewing wastewater and fermentation wastewater. Control strains: QX-1, QX-2, QX-3, and QX-4 are bacilli, which are also functional strains for degrading various organic acids, sugars, and alcohols; S-1 and S-2 are bacilli, which are for degrading Functional bacteria of petroleum; FA-1, FA-2, FA-3 are functional bacteria for treating oleochemical wastewater [Huang Jun, Bai Wei, Li Yijun. Isolation and identification of three strains of oleochemical wastewater degrading bacteria. Journal of Applied and Environmental Biology, 1999 , 5(Suppl): 74-76.]; B-1, B-2 are bacilli, which are coking wastewater degrading bacteria; N-1, N-2 are bacilli, which are denitrification bacteria; H-1, H-2 For the coccus, for the decyanidation bacteria. All the above strains are preserved by the Environmental Room of Chengdu Institute of Bi...

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Abstract

The present invention relates to molecular biological technology, and is especially molecular mark of functional bacterium QX-5 for treating organic industrial effluent. The present invention screens out DNA characteristic segment of QX-5, designs PCR amplification primer and converts the characteristic segment into stable and reliable SCAR mark. The mark is used in identifying and monitoring functional bacterium QX-5 in organic industrial effluent treating system fast and accurately.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to SCAR (sequence characterized amplified region) marker screening, fragment separation and cloning and molecular detection. Background technique [0002] QX-5 is a Brevibacterium used to treat a variety of organic industrial wastewater, and the applicant has been applied to high-concentration brewing wastewater and biological pesticide fermentation wastewater treatment projects. The current method of detecting its content in the reaction system is to use the traditional pure culture technology. This method has cumbersome steps and a large workload, and it is difficult to apply to the rapid and accurate monitoring of microbial functional strains in actual wastewater treatment projects. [0003] In recent years, some mature molecular biology techniques have been widely used in environmental microbiology, enabling people to avoid the traditional isolation and culture process and direc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/31C07H21/00C12Q1/68
Inventor 黄钧马欣荣李毅军刘华玲
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S