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Microelectrode for recording laboratory animal visual sense electrophysiological reaction

A technology for recording electrodes and experimental animals, applied in applications, eye testing equipment, medical science, etc., can solve problems such as large deviations in measurement results, poor contact, and easy drift, and achieve stable recording waveforms, low manufacturing costs, and repeatable good performance effect

Inactive Publication Date: 2009-04-01
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the linear electrode is inconvenient to place and fix in the animal's eye, it is easy to drift and interfere with the recording, so that the recorded waveform has poor repeatability and small amplitude, which directly affects the wide application of visual electrophysiology in experimental animals.
[0009] At the same time, due to the different size and curvature of the cornea of ​​the animal eye, the different eyelids, the shape of the recording electrode and the difference in the manufacturing process, it will greatly affect the recording results.
[0010] In addition, there has been no special reference electrode product suitable for animal visual electrophysiological testing. In actual experiments, a human reference electrode is usually used as a temporary replacement. Since the human reference electrode is a sucker-type electrode, it has more For small rodent experimental animals with hair (such as experimental mice or rabbits, etc.), its fixation is more difficult, and because it cannot be in close contact with the animal's skin, it will bring problems such as inconvenient fixation, poor contact, contact Problems such as large resistance and large deviation of measurement results also lead to poor repeatability and small amplitude of recorded waveforms, which also directly affect the wide application of visual electrophysiology in experimental animals

Method used

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  • Microelectrode for recording laboratory animal visual sense electrophysiological reaction
  • Microelectrode for recording laboratory animal visual sense electrophysiological reaction
  • Microelectrode for recording laboratory animal visual sense electrophysiological reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The recording electrode is a ring-shaped corneal contact electrode, the reference electrode and the ground electrode are needle-shaped electrodes, and the experimental animal is a rabbit.

[0044] Put methyl cellulose (or ofloxacin ointment) on the corneal surface of rabbit eyes. After corneal surface anesthesia, place double-circle contact electrode with inner / outer electrode diameter of 3mm / 5mm respectively, reference electrode and ground electrode needle Electrodes are pierced into the ears on the same side and the top of the head.

[0045] Using the above-mentioned micro-electrodes, in conjunction with the American LKC UTAS-2000 electrophysiological instrument, dual-channel recording of ERG, the recorded waveforms are as follows: Figure 4 Shown.

[0046] It can be seen from the figure that the a→b segment in the displayed waveform curve is basically free of interference clutter (that is, no steep spikes), and the waveform curve is smoother. The repetitive performance o...

Embodiment 2

[0048] The recording electrode is a ring-shaped corneal contact electrode, the reference electrode and the ground electrode are needle-shaped electrodes, and the experimental animal is a BN (Brown Norway) rat.

[0049] The recording electrode adopts a 0.8mm / 1.5mm double-circle contact electrode. After anesthetizing the animal, it is placed directly into the eyeball. The surface is dripped with saline or methylcellulose. The reference electrode and the ground electrode are needle electrodes, respectively, pierce the same side ears. And the top of the head.

[0050] Using the above-mentioned micro-electrodes, in conjunction with the American LKC UTAS-2000 electrophysiological instrument, dual-channel recording of ERG, the recorded waveforms are as follows: Figure 5 Shown.

[0051] It can be seen from the figure that the N1→O4 segment of the displayed waveform curve has no interference clutter, and the waveform curve is smoother, which has a good diagnostic and evaluation value.

[00...

Embodiment 3

[0054] The recording electrode, the reference electrode and the grounding electrode adopt a metal needle electrode with a 1ml stainless steel needle as the tip for injection, and the experimental animal is a Wistar rat.

[0055] The recording electrode was pierced into the subcutaneous connection between the two ears, and the reference electrode and ground electrode were pierced into the same ear and the top of the head respectively.

[0056] Get the FVEP record waveform as Figure 6 Shown.

[0057] It can be seen from the figure that the displayed waveform curve has no clutter interference and stable waveform, which embodies the advantages of the technical solution of convenient and accurate positioning, easy fixation, good contact, stable recording waveform, and good repetitiveness of the recorded waveform.

[0058] The rest is the same as in Example 1 or 2.

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Abstract

The invention discloses a minimized electrode in the physiological checking element domain, which consists of recording electrode and or reference electrode, wherein the recording electrode is composed of conductive electrode rod and electrode on one end of conductive rod; the electrode is ring-shaped metal corneal contacting electrode; or the recording electrode and or reference electrode is composed of insulating conductive electrode rod and electrode on one end of rod conduct; the electrode is needle-shaped metal electrode.

Description

Technical field [0001] The invention belongs to the field of physiological test instruments, and particularly relates to a device for measuring animal bioelectric signals. Background technique [0002] Visual electrophysiology technology is the only objective visual function detection method commonly used in ophthalmology clinics. Since its clinical application for more than half a century, its production and development are inseparable from the basis of animal experiments. [0003] The non-invasiveness, objectivity, and molecular level of visual electrophysiology technology under special conditions, as well as the hierarchical positioning and accuracy of the detection of retinal and visual path diseases, make this technology useful in ophthalmology clinics and even in neurology departments. There is a very important and irreplaceable role in related cross-over research. [0004] In the past 10 years, visual electrophysiology technology has advanced by leaps and bounds, and many ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B3/10
Inventor 吴晓平张皙宫媛媛吴星伟许迅
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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