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Use of short-small-rod like bacillus for preparing pig's infections pleuropneumonia biological preparation

A technology for Corynebacterium brevis and pleuropneumonia, which is applied in the directions of antibacterial drugs, drug combinations, bacterial antigen components, etc., can solve the problems of irrelevance and comparability between the use method, the application object and the use purpose, and achieves less toxic and side effects. , easy to cultivate effect

Inactive Publication Date: 2009-07-29
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent and the existing patents use the same bacterium, the method of use, object of application and purpose of use are not related to each other, so there is no comparison

Method used

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  • Use of short-small-rod like bacillus for preparing pig's infections pleuropneumonia biological preparation
  • Use of short-small-rod like bacillus for preparing pig's infections pleuropneumonia biological preparation
  • Use of short-small-rod like bacillus for preparing pig's infections pleuropneumonia biological preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Corynebacterium brevis aluminum gel adjuvant preparation

[0040] Dilute the strain of Corynebacterium pumilus with 2ml of normal saline, inoculate it in 4g minced meat medium, culture it anaerobically at 37°C for 5 days, transfer it to the sodium thioglycolate antioxidant medium, culture it anaerobically, and then transfer it to the liver dialysate Cultivate concentrated, anaerobic culture for 5 days, collect the bacteria, dilute it with normal saline to 3 billion to 5 billion / ml, add 20% aluminum gel adjuvant, and shake well for later use. Take 0.1ml and inject it into ordinary broth and minced meat medium respectively, and carry out the inspection of miscellaneous bacteria, and take another 1ml of the preparation, and inject 0.2ml / mouse intraperitoneally into 5 Kunming mice with a body weight of 18-22 grams. Observe for 1 week, carry out microscopic examination on ordinary broth and minced meat culture medium, except for the known small rod-shaped bacteria, no other ...

Embodiment 2

[0042] Corynebacterium brevis astragalus polysaccharide adjuvant freeze-dried powder

[0043]Take 10 grams of the Corynebacterium pumilus thallus prepared in Example 1, dilute it to 5 billion / ml with physiological saline under aseptic conditions, add 2% astragalus polysaccharide adjuvant, add 2% skimmed milk, 1% glucose, repeat Mix well, put into 5ml vaccine bottles, the volume of each bottle is 2ml, freeze at -20°C for 24 hours, then transfer to -70°C for 48 hours, and finally put it into a vacuum freeze dryer for freeze-drying, which can be used for prevention and treatment Lyophilizate of Corynebacterium brevis from porcine infectious pleuropneumoniae. Each bottle contains 10 billion Corynebacterium brevis. Store at -20°C for 2 years.

Embodiment 3

[0045] Prepare capsule preparation of the present invention

[0046] Take 20 grams of the anaerobic Corynebacterium pumilus freeze-dried powder prepared in Example 3, add 30 grams of medicinal starch as an excipient, mix well, and pack into capsules, each containing 200 mg of anaerobic Corynebacterium pumilus medicinal ingredients.

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Abstract

The present invention relates to the new medical application of Corynebacterium brevis, in particular provides the use of Corynebacterium brevis for preparing biological preparations of porcine infectious pleuropneumonia, and provides the biological preparations of Corynebacterium brevis. At the genetic level, the gene differences between the main serotype 1 and serotype 5 standard strains of APP bacteria were studied, the genome subtraction library and the mRNA subtraction library were constructed, and the main difference gene fragments were analyzed and homology comparison on the Internet. Regardless of the differential genes at the genome or mRNA levels, APP strains had many homologous gene segments with important functional genes of Corynebacterium brevis.

Description

technical field [0001] The invention relates to new medical applications of Corynebacterium brevis, in particular provides a use of Corynebacterium brevis for preparing biological preparations for preventing and treating porcine infectious pleuropneumonia, and belongs to the field of veterinary biopharmaceuticals. Background technique [0002] The porcine infectious pleuropneumonia involved in the present invention is caused by the infection of actinobacillus (A. pleuropneumoniae), and is a highly contagious and lethal respiratory infectious disease of pigs. It was reported for the first time by British Pattison etc. in 1957, and it has been widely popular all over the world in the 1980s. In recent years, it has been internationally recognized as one of the important infectious diseases that endanger the modern pig industry. It is listed as one of the main pig diseases in the United States, Denmark, and Switzerland. With the development of scale and intensification of aquac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/05A61P11/00A61P31/04
Inventor 韩文瑜雷连成吕爽
Owner JILIN UNIV