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Method for extracting ganglioside with biological activity from animal tissues

A technology of gangliosides, physiological activity, applied in the field of biological extraction

Inactive Publication Date: 2009-08-19
吴浩青
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its importance, many studies on extraction preparations have been carried out, but few have addressed the issue of preserving its physiological activity

Method used

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  • Method for extracting ganglioside with biological activity from animal tissues

Examples

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Comparison scheme
Effect test

Embodiment 1

[0020] Wash 100 grams of pig brain in ice water, rinse it with deionized water, stir it into a slurry with a pulverizer, add 200ml of acetone pre-cooled to 4°C for extraction, filter it with a Buchner funnel, and use the same amount of acetone to filter the cake Process once and drain. Add 50ml of sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution to the obtained filter cake, stir well, add 200ml of cold tetrahydrofuran for extraction, and suction filter with a Buchner funnel. The residue is treated once in the same way. The filtrates were combined and placed in a refrigerator at -17°C to freeze to precipitate an ice crystal solid (A), and the liquid phase (B) was separated and removed. (A) was distilled under reduced pressure at 35°C, concentrated to 80ml, added 400ml of cold acetone, stood overnight in the refrigerator, filtered to obtain a white solid, and dried in vacuum at -17°C to obtain 7-9g of crude product.

Embodiment 2

[0022] Wash 4 pig brains in ice water, rinse them with deionized water, grind them into a slurry with a pulverizer, add 250ml of acetone pre-cooled to -5°C for extraction, and filter twice (400ml acetone). Add 50 ml of citric acid-disodium hydrogen phosphate buffer solution to the residue, stir well, add 250 ml of cold tetrahydrofuran for extraction, filter, and repeat this process once to obtain 600 ml of filtrate. The filtrate was frozen in a -20°C refrigerator until all ice crystals were precipitated, and the ice cubes were taken out and distilled under reduced pressure at 30°C to concentrate from about 400ml to about 80ml. Add 320ml of cold acetone at 1°C to precipitate a solid, separate the solid, and dry it under vacuum at -17°C to obtain a white solid product.

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Abstract

The invention discloses a method for extracting ganglioside from animal tissue. It is very important to extract useful substances from biomass and maintain their original physiological activity. This method uses acetone, tetrahydrofuran, methanol, chloroform and other polar solvents to extract gangliosides from animal tissues under the condition of maintaining a certain acidity in a buffer solution containing Na2HPO4, NaH2PO4, or citric acid. The whole process uses ion Exchange water (without heavy metals), and keep the treatment temperature below 35°C to avoid or reduce the loss of its physiological activity. The obtained product is a crude product of ganglioside, which contains some other glycolipids besides ganglioside, and has health care function.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, in particular to a method for extracting gangliosides from animal tissues. technical background [0002] Gangliosides are a group of closely related glycosphingolipids characterized by the presence of sialic acid (N-acetylneuraminic acid), composed of hydrophilic sialic acid-containing oligosaccharide chains and hydrophobic ceramides ( figure 1 ). According to the number of sialic acid in the molecule, it can be divided into G M (monosialoganglioside), G D (disialoganglioside) and G T (trisialoganglioside); press G M The number of glycosyl groups can be divided into G containing 4 glycosyl groups M1 , G of 3 sugar groups M2 G with 2 sugar groups M3 . Since Klenk first discovered it in 1935, more than 70 species have been isolated and identified. G M1 It is the main species of mammalian gangliosides. [0003] Gangliosides are an important component of biological membranes ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/10C07H1/08
Inventor 吴浩青冯薇荪吴文辉吴杰
Owner 吴浩青
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