Method for separating polysaccharide against growth of cancer cells from dictyophord

A technology of Radix Rhododendron, a separation method, applied in the directions of antitumor drugs, organic active ingredients, medical preparations containing active ingredients, etc., can solve problems such as waste

Inactive Publication Date: 2009-08-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the processing of the finished product of Dictyophora edulis, producers often use its stipe and fungus part, and remove the cap and fungus as leftovers (the ratio of the weight of the stipe and fungus to the weight of the cap and the fungus 1:1.3), resulting in a great waste, studies have shown that the dried mushrooms are rich in polysaccharides, amino acids, proteins, vitamins and beneficial metal elements
But so far, there is no report on polysaccharides that can inhibit the growth of tumor cells on Dictyophora edulis.

Method used

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  • Method for separating polysaccharide against growth of cancer cells from dictyophord
  • Method for separating polysaccharide against growth of cancer cells from dictyophord
  • Method for separating polysaccharide against growth of cancer cells from dictyophord

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Experimental program
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Embodiment Construction

[0022] Isolation of polysaccharides from Dictyophora edulis

[0023] (1) After drying and crushing the fungus receptacle of Dictyophora edulis, leaching with 15 times the volume of water for 4 hours, filtering, concentrating the filtrate to 50% of the original volume, adding ethanol for alcohol analysis, separating the precipitate and to dry, to obtain dry matter;

[0024] (2) Deproteinization by Sevag method

[0025] The dried product was made into an aqueous solution, and an equal volume of Sevag agent (chloroform: isoamyl alcohol = 3:1, volume ratio) was added to the aqueous solution and shaken on a shaker for 4 hours, then centrifuged and separated to obtain an organic phase and an aqueous phase. By this method, the effect of protein removal is obvious, not only the removal rate is high, but also the process is simple, no need for large power and equipment, and the operating cost is greatly reduced.

[0026] (3) adding ethanol to the aqueous phase obtained in step (2) fo...

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Abstract

The invention discloses a method for separating polysaccharides that can inhibit the growth of tumor cells from the fungus receptacle of Dictyophora edulis. The dried precipitate is made into an aqueous solution, deproteinized by the Sevag method, followed by alcohol analysis, separated and dried, and made into a 12mg / mL aqueous solution, which is separated in a chromatographic column to obtain the polysaccharide of Dictyophora edulis DRVP1. The polysaccharide DRVP1 obtained by the method of the invention has obvious inhibitory effect on the growth of tumor cells.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides from edible fungi, in particular to a method for separating polysaccharides contained in the fungus receptacle of Dictyophora edulis. Background technique [0002] Malignant tumors are major refractory diseases that seriously endanger human health, and drug therapy is still an important means of tumor prevention and treatment. my country is rich in medicinal plant resources, and the use of natural drug resources to develop new anti-tumor drugs with independent intellectual property rights has broad prospects. [0003] Dictyophora rubrovalvata is a basidiomycete belonging to Eumycota, Basidiomycotina, Gasteromycetes, Phallales, Phallaceae, Dictyophora (Dictyophora), the fruiting body of Dictyophora edulis is 20-33cm high; the buds are oval, dark red, 4-5cm×4-6cm in size, the rhizomes are white, and will turn blue-purple after injury; The receptacle is dark red, the cap is bell-shaped or ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 王飞娟潘薛波赵凯朱诚高建青陈文莉
Owner ZHEJIANG UNIV
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