Anti-cancer Tengli root extract and its making method and use
An anti-tumor and extract technology, applied in the field of medicine, can solve the problems of separation of active components and application of tumor treatment, no relevant patents, no effective parts of vine pear root for anti-tumor, method of separation of components, preparation technology, etc., so as to facilitate industrialization. The effect of good production and extraction effect and high transfer rate
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Embodiment 1
[0031] Add 8L of water to 1Kg of rattan pear root, keep it at 100°C for 60 minutes, then filter to obtain the first leachate; then add 4L of water to the filter residue, keep it at 100°C for 60 minutes Minutes, then filter to obtain the second leachate; combine the two leachates.
[0032] Concentrate the leaching solution to a thermal specific gravity of 1.05 (5L), adjust the pH value of the leaching solution to 2 with 10% hydrochloric acid, then let stand, centrifuge to remove the precipitate, and obtain the supernatant; use 5L of ethyl acetate to extract the supernatant twice, Combine the extracts, recover ethyl acetate, and obtain the extract; dissolve the extract with an equal volume of water, load it on a polyamide column for chromatography, and then use 30-70% ethanol for gradient elution, and collect and elute in sections solution; finally, the 70% ethanol eluate was concentrated under reduced pressure, the ethanol was recovered, and then vacuum-dried to obtain 28 g of ...
Embodiment 2
[0034] Grind 1Kg of vine pear root medicinal material into coarse powder, add 9L of water, keep at 90°C for 90 minutes, then filter to obtain the first leachate; then add 5L of water to the filter residue, keep at 90°C Under leaching for 90 minutes, and then filtered to obtain the second leaching solution; the two leaching solutions were combined.
[0035] Concentrate the leachate to a thermal specific gravity of 1.05 (6L), adjust the pH value of the leachate to 3 with 5% hydrochloric acid, then let stand, centrifuge to remove the precipitate, and obtain the supernatant; extract the supernatant twice with 12L of ethyl acetate, Combine the extracts, recover ethyl acetate, and obtain the extract; dissolve the extract with an equal volume of ethanol, mix the sample with 2 times the weight of polyamide, load the sample on a polyamide column for chromatography, and then use 0-50% ethanol for Gradient elution, and the eluate was collected in sections; finally, the 50% ethanol eluate...
Embodiment 3
[0037] Grind 1Kg of vine pear root medicinal material into coarse powder, add 10L of water, keep at 80°C for 120 minutes, then filter to obtain the first leachate; then add 5L of water to the filter residue, keep at 80°C Extract at low temperature for 120 minutes, then filter to obtain the second leach solution; then add 5 L of water to the filter residue, keep it at 80°C for 120 minutes, then filter to obtain the third leach solution; combine the three leach solutions.
[0038] Concentrate the leachate to a thermal specific gravity of 1.03 (10L), adjust the pH value of the leachate to 5 with 20% acetic acid, then let stand, centrifuge to remove the precipitate, and obtain the supernatant; extract the supernatant once with 10L of ethyl acetate, Combine the extracts, recover ethyl acetate, and obtain the extract; dissolve the extract with an equal volume of water, load it on a polyamide column for chromatography, and then use 30-80% ethanol for gradient elution, and collect the ...
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