H9 sub-type fowl-flu HI stabilized antigen

Abstract

The invention provides a H9 subtype avian influenza HI stable antigen, which is obtained from inactivated virus, and its manufacturing process is as follows: add an inactivator according to 0.1-0.2% of the volume of the virus liquid, and inactivate it at 4°C 16 to 24 hours; concentrate the inactivated virus liquid to 1 / 2 to 1 / 5 of the original volume, and then use ultrasonic waves to treat the virus liquid; Bacteria stabilizer, can be used after shaking and mixing. The inactivated antigen does not have the danger of releasing poison during use and storage, reduces the harm to the human body caused by the use of the antigen, and has good potency at the same time, and will not affect the accuracy of detection.

Description

technical field [0001] The invention relates to biotechnology, in particular to a H9 subtype avian influenza HI stable antigen. Background technique [0002] Avian influenza (Avian Influenza, AI) is a severe infectious disease of poultry caused by influenza virus of Orthomyxoviridae family. It is a syndrome of various diseases ranging from respiratory system to severe systemic sepsis. Can infect a variety of birds: poultry (such as chickens, ducks, geese, turkeys, pigeons, etc.), rare birds (such as guinea fowl, swans, gulls, etc.), ornamental birds (such as parrots, wrens, etc.) , ducks, terns, etc.) etc. At present, there are as many as 88 species of poisonous birds that have been documented. According to the different pathogenicity, it can be divided into highly pathogenic, low pathogenic and non-pathogenic avian influenza. Low-pathogenic avian influenza (such as H9 subtype) can seriously affect the production performance of poultry, and the egg production rate can dro...

AI Technical Summary

Problems solved by technology

However, the primary problem of the current HI detection method is the lack of a stable and safe HI diagnostic antigen

The specific manifestations are as follows: firstly, the hidden danger of the live virus antigen is great. Since the avian influenza diagnostic antigen used by the existing units is a live virus, there must be a danger of loosening the virus; secondly, the live virus antigen must be frozen to protect the activity of the virus itself, which requires high requirements. If placed at 4°C, protein precipitation will begin to occur within one week, and the titer will decrease, and repeated freezing will denature the protein and reduce the antigen titer; in addition, the stability is poor, and the use of live viruses as diagnostic antigens needs to be tested every time. Determination of the hemagglutination titer of the antigen increases the workload; although the live virus can be inactivated to reduce the risk of virus spread, the prior art generally uses formaldehyde as an inactivator, and formaldehyde inactivation is generally the most effective This will destroy the spike protein on the surface of the virus, and the spike protein is indispensable for hemagglutination tests. The destruction of a large number of surface spike proteins will reduce the hemagglutination titer Moreover, the storage time is greatly shortened, and the stability of the viral hemagglutinin after simple inactivation is poor. At the same time, it is greatly affected by factors such as storage conditions, buffers and inactivators, which will also lead to a sharp decrease or even disappearance of the hemagglutination titer. It can be seen that The antigens currently used in practice are all made from live viruses, and formaldehyde is not suitable for the inactivation of this virus as an inactivating agent.

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