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Method for artificially breeding edulis mono-garlic clove orchid

A kind of artificial propagation technology of Arthia chinensis alone garlic orchid, which is applied in horticultural methods, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of undiscovered cost, short growth cycle, difficult seed germination, etc., and achieve the solution of artificial Technical difficulties in reproduction, short growth cycle, and high survival rate of seedlings

Inactive Publication Date: 2010-12-22
SOUTHWEST JIAOTONG UNIV
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  • Summary
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  • Description
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AI Technical Summary

Problems solved by technology

However, in the natural state, it is extremely difficult to germinate the seeds of Arthia sativa L., and there is no artificial propagation method for the seedlings of Arthia sativa L. with low cost, short growth cycle and high survival rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for artificial propagation of Arthia chinensis L., comprising the following steps:

[0024] a. Material selection: select Arthias chinensis L. solifera plants with excellent properties, artificially pollinate them after flowering, and usually bear fruit after 120 days. After the results were obtained, the uncracked fruit pods were collected, and the surface of the pods was wiped with filter paper. After vacuum sealing, they were placed in a refrigerator at 4°C for cold treatment for 1 month.

[0025] b. Induce germination: take out the fruit pods in step a from the refrigerator for routine sterilization, cut open the fruit pods, and spread the powdered seeds in it on the semi-solid acidic medium; culture in the dark at 25±1°C in the culture room , so that the seeds expand, the embryo breaks through the seed coat, and gradually forms a small white transparent sphere. This process generally takes 3 months. Generally, after another half a month, it transforms int...

Embodiment 2

[0032] A method for artificial propagation of Arthia chinensis L., comprising the following steps:

[0033] a. Material selection: select Arthia chinensis L. solifera plants with excellent traits, artificially pollinate them after flowering, collect brown-yellow uncracked fruit pods after fruiting, wipe the surface of the fruit pods with filter paper, vacuum-seal them and place them in a refrigerator at 4°C Cold treatment for 0.5 months.

[0034] b. Induce germination: take out the fruit pods in step a from the refrigerator for routine sterilization, cut open the fruit pods, and spread the powdered seeds in it on the semi-solid acidic medium; culture in the dark at 25±1°C in the culture room , so that the seed expands, the embryo breaks through the seed coat, and gradually forms a small white transparent sphere, and then transforms into a dense protosphere, and finally gradually forms a protocorm.

[0035] The semi-solid acidic medium used in this example is: add 1mg / L 2,4-di...

Embodiment 3

[0040] A method for artificial propagation of Arthia chinensis L., comprising the following steps:

[0041] a. Material selection: select Arthia chinensis L. solifera plants with excellent traits, artificially pollinate them after flowering, collect brown-yellow uncracked fruit pods after fruiting, wipe the surface of the fruit pods with filter paper, vacuum-seal them and place them in a refrigerator at 4°C Cold treatment for 2 months.

[0042] b. Induce germination: take out the fruit pods in step a from the refrigerator for routine sterilization, cut open the fruit pods, and spread the powdered seeds in it on the semi-solid acidic medium; culture in the dark at 25±1°C in the culture room , so that the seed expands, the embryo breaks through the seed coat, and gradually forms a small white transparent sphere, and then transforms into a dense protosphere, and finally gradually forms a protocorm.

[0043] The semi-solid acid medium used when inducing germination is: add 3mg / L ...

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Abstract

An artificial reproduction method for appendiculate cremastra and common pleione includes such steps as choosing excellent seeds, low-temp treating, dark culturing in semi-solid acidic medium at 25 + / - 1 deg.C for inducing germination, dark culturing in differentiating culture medium, secondary culturing in illuminescent condition, irradiating by natural light, and transplanting in matrix in plastic tray.

Description

technical field [0001] The invention belongs to the technical field of artificial propagation of plants, in particular to the technical field of artificial propagation of the endangered medicinal orchidaceae Arthrowia chinensis. Background technique [0002] Arthrowia chinensis Du Suanlan is the basic plant of the crude drug Arthrowia shanthii. Shanzigu is a commonly used traditional Chinese medicine, which has the functions of clearing away heat, resolving phlegm, detoxifying, eliminating carbuncle and dispelling stagnation. It is clinically used to treat carbuncle, swollen furunculosis, scrofula, sputum nucleus, lymph node tuberculosis, malignant sore, snake bites and other diseases. In recent years, Arrowhead Arrowhead has achieved certain curative effects in the treatment of gastric cancer and other cancers, so it has attracted much attention as a new anticancer drug. In vitro experiments have shown that Arthia chinensis has inhibitory effects on both transplanted mous...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01C1/00A01H4/00C12N5/04A01G31/00
Inventor 王万军冯佛生杜长洪赵鹏杨中奇
Owner SOUTHWEST JIAOTONG UNIV
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