Animal feed additives based on fermentation broth and production process thereof by granulation
A technology of animal feed and production method, applied in the forming or processing of animal feed, animal feed, animal feed, etc., can solve the problems of increasing difficulty, achieve the effects of reducing risk, stabilizing lysine content, and increasing content
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Embodiment 1
[0065] [Example 1] directly form granule by fermented liquid
[0066] In this example, Corynebacterium glutamicum CJM107 (KCCM-10227) was prepared with 1 l of process water as a standard, in a mixture containing 50 g of raw sugar, 10 g of peptone, 10 g of yeast extract, 5 g of urea, and KH 2 PO 4 4g,K 2 HPO 4 8g, MgSO 4 ·7H 2 O 0.5g, biotin 100μg, thiamine HCl 1,000μg of medium 18l, cultured at a culture temperature of 35°C and medium pH 6 to 8 for 98 hours to complete the lysine fermentation process. The lysine content in the fermentation broth was 18%.
[0067] After concentrating the filtrate of the fermented broth to a level of 55.2 wt% of the total solid content by concentrating under reduced pressure, the pH is adjusted to 3.5-3.6. The pH adjustment was performed with sulfuric acid, and after the pH adjustment, the concentrated solution was left in a 60° C. environment for 2.5 hours.
[0068] After the gDNA decomposition process, the concentrated liquid was injec...
Embodiment 2
[0070] [Example 2] Adding free lysine in the fermentation broth to adjust the content
[0071] The fermented liquid (with a lysine content of 14.5%) produced through the fermentation process under the same conditions as in Example 1 was concentrated to a total solid content of 51.5 wt%. 8 g of free lysine (manufactured by CJ Co., Ltd.) was added thereto, and after mixing in a mixing box, the same conditions as in Example 1 were used to prepare granules.
[0072] The finally obtained product has a lysine content of 68%, a protein content of 14%, a moisture content of 0.13%, and a surface density of 665.5kg / m 3 , Inorganic matter content 2.50%, particle size range 0-500μm or less is 3%, more than 500μm and 1000μm is 28%, 1000-1300μm is 67%, more than 1300μm is 2%.
Embodiment 3
[0073] [Example 3] Adding excipients in the fermentation broth to adjust the content
[0074] The fermented liquid produced by fermentation under the same conditions as in Example 1 was used, and its lysine content was 20%, and the total solid content was 25.8%.
[0075] After the fermentation broth was concentrated, an excipient with a moisture content of 9.0% prepared by dissolving 0.22 kg of cornstarch in 0.5.L of water was added to the concentrated solution as a content regulator, and mixed in a mixing box.
[0076] Concentrate the filtrate of the fermented broth to a solid content of 50.5 wt% by concentrating under reduced pressure, and then make granules under the same conditions as in Example 1.
[0077] In this example, the finally obtained product has a lysine content of 66%, a protein content of 14%, a moisture content of 0.24%, and a surface density of 682.5kg / m 3 , Inorganic matter content 1.88%, particle size range 0-500μm or less is 4%, more than 500μm and 1000μ...
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