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Method for controlling the quality of pine pollen fingerprinting in the resistance-strengthening and stasis-dispersing plant medicine

A quality control method and fingerprint technology, which are applied in the field of quality control of pine pollen raw materials, can solve problems such as no literature reports, and achieve the effects of strong specificity, reliable method and easy operation.

Active Publication Date: 2007-09-26
SHANGHAI MODERN CHINESE TRADITIONAL MEDICINE TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature report on the research on the fingerprint of pine pollen

Method used

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  • Method for controlling the quality of pine pollen fingerprinting in the resistance-strengthening and stasis-dispersing plant medicine
  • Method for controlling the quality of pine pollen fingerprinting in the resistance-strengthening and stasis-dispersing plant medicine
  • Method for controlling the quality of pine pollen fingerprinting in the resistance-strengthening and stasis-dispersing plant medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Weigh 1.0g of pine pollen, add 100ml of 80% methanol, extract under reflux for 2h, filter, spin the filtrate to dryness, use 50% methanol to make up the volume in a 25ml volumetric flask, and pass through a 0.45μm microporous membrane to obtain the test product. Chromatographic conditions: column temperature: 30°C, detection wavelength: 260nm, injection volume: 20μl, chromatographic column: alltima (250mm×4.6mm, 5μm). See Table 1 for elution conditions

[0044] time (min)

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PUM

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Abstract

The invention relates to a fingerprint quality control method of Chinese medicine drug pine pollen, comprising that (1), preparing pine pollen sample, (2), washing out flow phase gradient, (3), building standard fingerprint that using naringinase as index peak, (4), controlling the quality of fingerprint that a, checking the sample, while the held time of each characteristic peak is that the first peak is held for 0.20, the third peak is held for 0.44, the fourth peak is held for 0.48, the fifth peak is held for 0.97, and the sixth peak is held for 1.00, b, evaluating sample quality that comparting the HPLC fingerprint of sample with standard fingerprint (or reference share model fingerprint), while the similarity of total seven peaks are not lower than 0.9, (5), the pine pollen collection method. The invention can completely control the material quality to make product in stable quality.

Description

technical field [0001] The invention belongs to the field of quality control methods of pine pollen raw materials, in particular to a method for quality control of fingerprints of pine pollen raw materials in herbal medicines for strengthening health and removing blood stasis. Background technique [0002] Pine pollen (Pollen Pini) is the dried pollen of Pinus massoniana Lamb., a plant of the Pinaceae family. Synonymous pine yellow, pine powder. my country's earliest pharmacy monograph "Shen Nong's Materia Medica" listed pine pollen (pine yellow) as the top grade, saying that pine pollen "smells sweet and flat and non-toxic. Yannian. "Newly Revised Materia Medica", said: "The name of the pine flower is Songhuang, and it looks like Puhuangzhenger when you brush it. "Compendium of Materia Medica" also contains pine pollen. [0003] "Flora of China" describes Masson pine as an arbor with a height of 45m and a diameter at breast height of 1.5m. Bark reddish brown, lower part ...

Claims

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Application Information

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IPC IPC(8): A61K36/15A61P13/00A61P43/00G01N30/02G01N30/86A61K133/00
Inventor 胡坪潘一峰
Owner SHANGHAI MODERN CHINESE TRADITIONAL MEDICINE TECH DEV
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