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Method of producing acid hydrolyzed casein

A technology of acid hydrolysis casein and production method, which is applied in the field of organic chemical acid hydrolysis, can solve the problems of inability to carry out industrial production, long hydrolysis time, low output rate, etc., achieve shortened production cycle, short hydrolysis time, and reduce production cost Effect

Active Publication Date: 2008-01-02
临夏州华安生物制品有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the long hydrolysis time, high energy consumption and low output rate, this kind of production process can only be used by researchers to make a small sample for research in the laboratory, and it cannot be industrialized at all.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1 Hydrolysis

[0038] Accurately weigh 100g of casein and add it to a 1000ml three-necked bottle, then add 100ml of distilled water and stir evenly, 100ml of 12mol / L concentrated hydrochloric acid and stir evenly, install a condenser, electric stirring, and electric heating mantle, and heat up while stirring. When the solution reaches 90°C, the solution boils as a whole, and the hydrolysis is continued with constant temperature stirring for 3 hours, and the hydrolysis is stopped after the solution is completely hydrolyzed without casein particles.

[0039] 2 filters

[0040] Add 100ml of distilled water to the hydrolyzed solution hydrolyzed in the first step, and filter it with an 80-mesh nylon mesh.

[0041] 3 concentrated

[0042] Add the above-mentioned filtrate to a 1000ml round bottom flask in portions, reduce the pressure to 650mmHg with a rotary evaporator and concentrate to a paste, add 100ml of distilled water through negative pressure, reduce the rotation sp...

example 2

[0050] 1 hydrolysis

[0051] Accurately weigh 200g of casein and add it to a 1000ml three-neck bottle, then add 400ml of distilled water and stir evenly, 400ml of 4mol / L sulfuric acid and stir evenly, install a condenser, electric stirring, and electric heating mantle, and heat up while stirring. When the temperature reaches 100°C, the solution boils as a whole, and the hydrolysis is continued with constant temperature stirring for 3 hours, and the hydrolysis is stopped after the solution is completely hydrolyzed without casein particles.

[0052] 2 filters

[0053] Add 400ml of distilled water to the hydrolyzed solution that has been hydrolyzed in the first step, and filter it with a 120-mesh nylon mesh.

[0054] 3 concentrated

[0055] Add the above-mentioned filtrate to a 1000ml round bottom flask in batches, reduce the pressure to 700mmHg with a rotary evaporator and concentrate to a paste, add 600ml of distilled water through negative pressure, reduce the rotation speed...

example 3

[0063] 1 Hydrolysis

[0064] Accurately weigh 200g of casein and add it to a 1000ml three-neck bottle, then add 200ml of distilled water and stir evenly, 200ml of 6mol / L phosphoric acid and stir evenly, install a condenser, electric stirring, and electric heating mantle, and heat up while stirring. When the solution reaches 95°C, the solution boils as a whole, and the hydrolysis is continued for 3 hours with constant temperature stirring, and the hydrolysis is stopped after the solution is completely hydrolyzed without casein particles.

[0065] 2 filters

[0066] Add 100ml of distilled water to the hydrolyzed solution that has been hydrolyzed in the first step, and filter it with a 100-mesh nylon mesh.

[0067] 3 concentrated

[0068] Add the above filtrate to a 1000ml round bottom flask in batches, reduce the pressure to 700mmHg with a rotary evaporator and concentrate to a paste, add 200ml of distilled water through negative pressure, reduce the rotation speed of the rota...

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PUM

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Abstract

The invention discloses a method for producing acid-hydrolytic casein, which takes milk coarse protein that is used to extract butter as raw material, and comprises following steps: selecting, disintegrating, dissolving, filtering, acidifying, washing, dewatering, drying, purifying and getting casein, hydrolyzing with 4-12mol / l hydrochloride or sulfate or phosphoric acid at 90-100 Deg. C for 2-4 hours, condensing and washing repeatedly for 1-3 times, de-coloring with active carbon, exchanging with weak alkali or weak anion resin, spray drying at 120-160 Deg . C, and getting acid-hydrolytic casein. The invention is characterized by short hydrolysis time, simple process, short time for whole process of about 8-10 hours, reduced production period and cost, and suitability for mass industrial production.

Description

technical field [0001] The invention relates to the field of organic chemical acid hydrolysis, in particular to a production method for producing acid hydrolyzed casein by using milk crude protein extracted from ghee as a raw material. Background technique [0002] At present, in domestic research fields, the hydrochloric acid concentration is 18%, the solid-liquid ratio is 1:1.6, the hydrolysis temperature is 106°C, the hydrolysis time is 18 hours, and after the hydrolysis is completed, the pH value is adjusted to 4.9-5.2 with sodium hydroxide solution, and then decolorized with activated carbon. The best reaction conditions to prepare acid hydrolyzed casein, the specific process is: casein + 18% hydrochloric acid solution (1:1.6) heated to 106 ° C → hydrolyzed for 18 hours → filtered → adjust the pH value to 4.9-5.2 with sodium hydroxide solution → Resin exchange, the first step: strong acidic cation 001×7 (732), the second step: strong basic anion 201×7 (717) → concentrat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/10A23J3/32
Inventor 马志安
Owner 临夏州华安生物制品有限责任公司
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