Method for detecting content of sialic acid in milk powder or whey powder

A detection method, whey powder technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of sialic acid content in unpublished whey powder and formula milk powder, achieve high accuracy, improve detection efficiency, and operate simple effect

Pending Publication Date: 2022-05-13
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, my country has not promulgated the standard method for the detection of sialic acid content in whey powder and formula milk powder

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting content of sialic acid in milk powder or whey powder
  • Method for detecting content of sialic acid in milk powder or whey powder
  • Method for detecting content of sialic acid in milk powder or whey powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] In this embodiment, a commercially available whey powder rich in casein glycomacropeptide (hereinafter referred to as CGMP), α-lactalbumin powder, and whey powder rich in milk fat globule membrane (hereinafter referred to as MFGM) were selected to conduct saliva Acid content detection. The specific method is as follows:

[0054] (1) Reagents:

[0055] Water: Class I water in accordance with GB / T 6682;

[0056] Sulfuric acid: superior grade;

[0057] Anhydrous sodium acetate: superior grade;

[0058] 50% sodium hydroxide: chromatographically pure.

[0059] (2) Sample pretreatment:

[0060] Accurately weigh 0.1g (accurate to 0.0001g) of whey powder rich in casein glycomacropeptide, dissolve it in water and set the volume in a 10ml volumetric flask, vortex and shake well; take 1mL of the dissolved liquid in a 15mL centrifuge tube In the process, use sulfuric acid solution (0.05mol / L) to fix the volume to 10mL; place the prepared hydrolyzate in a constant temperature ...

Embodiment 2

[0086] In this example, a commercially available brand of infant formula powder 1, infant formula powder 3 and whole milk powder were selected for the detection of sialic acid content. The specific method is as follows:

[0087] (1) Reagents:

[0088] Water: Class I water in accordance with GB / T 6682;

[0089] Sulfuric acid: superior grade;

[0090] Sodium chloride, sodium acetate, 50% sodium hydroxide: all chromatography grade.

[0091] Sample pretreatment:

[0092]Accurately weigh 1g of the milk powder sample (accurate to 0.0001g), dissolve it in water and dilute it in a 10mL colorimetric tube, and vortex to mix. Take 2 mL of the dissolved liquid sample in a 15 mL centrifuge tube, and dilute to 10 mL with sulfuric acid solution (0.05 mol / L). Place the prepared milk powder hydrolyzate in a constant temperature water bath and adjust the temperature of the water bath. During this process, use a mercury thermometer inserted into the hydrolyzate for actual measurement until ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Elution gradientaaaaaaaaaa
Elution gradientaaaaaaaaaa
Login to view more

Abstract

The invention provides a method for detecting the content of sialic acid in milk powder or whey powder, which comprises the following steps: dissolving milk powder or whey powder in water to obtain an aqueous solution; adding an acid solution to the aqueous solution to obtain a milk powder acid solution or a whey powder acid solution; heating the milk powder acid solution or the whey powder acid solution for hydrolysis to obtain milk powder hydrolysate or whey powder hydrolysate; performing centrifugal separation on the milk powder hydrolysate or whey powder hydrolysate to obtain a milk powder supernatant or a whey powder supernatant; diluting the whey powder supernatant, and filtering to obtain whey powder filtrate; or carrying out solid-phase extraction on the milk powder supernatant, leaching with water, eluting with a sodium chloride solution, and collecting to obtain a milk powder eluent; diluting and filtering the whey powder filtrate or the milk powder eluent to obtain filtrate; and determining the filtrate by using a high performance anion exchange chromatography-electrochemical detector. The method disclosed by the invention is simple to operate, and can be used for accurately, qualitatively and quantitatively analyzing N-acetylneuraminic acid in whey powder and milk powder.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a method for detecting residual sialic acid in milk powder or whey powder. Background technique [0002] Sialic acid (SA) is a family of nitrogen- or oxygen-substituted derivatives of neuraminic acid, which widely exists in a variety of biological tissues and is an important component of glycoproteins and glycolipids on cell membranes. The sialic acid found in most mammalian tissues is mainly N-acetylneuraminic acid (Neu5Ac), see formula 1, and N-acetylneuraminic acid is usually called sialic acid in a narrow sense. Sialic acid is involved in a variety of physiological functions on the cell surface, and is also a natural brain nutrient that plays an important role in the immune system and cognitive development of infants and young children. Studies have found that the total sialic acid content in bovine milk is less than 25% of the total sialic acid in human m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/96G01N30/06G01N30/64
CPCG01N30/96G01N30/06G01N30/64G01N2030/047
Inventor 武伦玮段国霞唐烁胡雪刘丽君李翠枝
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap