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Usage, method and reagent case for prediction of cardio-cerebrovascular disease occurrence by polymorphism site genetype

A cardiovascular and cerebrovascular disease, polymorphic locus technology, applied in the field of medicine, can solve problems such as adverse outcomes for patients, and achieve the effects of reducing medical costs, good prognosis, and preventing the occurrence of cardiovascular and cerebrovascular diseases.

Inactive Publication Date: 2008-01-02
ANHUI PROV INST OF BIOLOGICAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The intervention and treatment of some known and recognized risk factors such as hypertension, hyperlipidemia and hyperglycemia have a certain effect on the prevention of cardiovascular and cerebrovascular diseases, but there are still some patients who have adverse effects due to the existence of other unknown risk factors. as a result of

Method used

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  • Usage, method and reagent case for prediction of cardio-cerebrovascular disease occurrence by polymorphism site genetype
  • Usage, method and reagent case for prediction of cardio-cerebrovascular disease occurrence by polymorphism site genetype

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Determine the C677T (Ala222Val, dsSNP ID: rs1801133) polymorphism site genotype of the MTHFR gene and predict the occurrence and prognosis of cardiovascular and cerebrovascular diseases

[0069] (1) Determination of the C677T polymorphism site genotype of the MTHFR gene

[0070] (1) Extract the genomic DNA of the host cell

[0071] (a) Add 30ml of erythrocyte lysate to the whole blood, shake slowly, and let stand at room temperature for 10 minutes. During this period, shake several times to completely lyse the erythrocytes;

[0072] (b) Centrifuge at 4°C and 2000 rpm for 10 minutes, remove the supernatant, break up the precipitated white blood cells on a rotary shaker, add 40 μl of protease and 50 μl of RNase, shake well, add 15 ml of white blood cell lysate, and mix Take it out after evenly bathing in 37°C water bath for 20 minutes, and put it in cold water;

[0073] (c) Add 4ml of cold protein precipitation solution, mix well and put it in the refrigerato...

Embodiment 2

[0145] Example 2: A kit for determining the genotype of the MTHFR C677T polymorphism site and predicting the occurrence and prognosis of cerebrovascular diseases

[0146] (1) The components of the kit: nucleic acid extraction reagents, PCR reaction reagents, MTHFR C677T polymorphic site genotype-specific primers, nucleic acid polymerase, restriction endonucleases, enzyme digestion reaction mixture and positive control template, Negative control templates were subpackaged and assembled into kits. The positive control templates include MTHFR C677T homozygous wild-type, heterozygous, and homozygous mutant positive control templates; the specific primers are primers capable of specifically amplifying at least the polymorphic site containing MTHFR C677T.

[0147] (2) Detection steps:

[0148] (1) Extract the genomic DNA of the host cell:

[0149](a) Add 400 μl of erythrocyte lysate to a 1.5ml centrifuge tube, and add about 100 μl of fresh whole blood or anticoagulated whole blood...

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Abstract

The invention discloses a polymorphism site genetype of MTHFR gene to predict individual cardiovascular disease and prognostic application in the medical domain, which detects polymorphism parting oligonucleotide of MTHFR gene and polymorphism site genetype through MTHFR gene to predict the individual cardiovascular disease and prognostic method and agent box.

Description

technical field [0001] The invention relates to the use of the polymorphic site genotype of the methylenetetrahydrofolate reductase (MTHFR) gene for predicting the occurrence and prognosis of cardiovascular and cerebrovascular diseases. The invention also relates to a method and a kit for predicting the occurrence and prognosis of cardiovascular and cerebrovascular diseases by measuring the genotype of the polymorphic site of the MTHFR gene. The invention belongs to the field of medicine. Background technique [0002] Stroke has four characteristics of high incidence, high recurrence rate, high disability rate and high mortality rate, and the treatment methods are limited. It is one of the most serious diseases that endanger human health. Therefore, the prevention of various risk factors is becoming more and more important. important. Risk factors for stroke can be divided into two parts: controllable and uncontrollable. Controllable risk factors are the primary preventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/50
Inventor 徐希平邢厚恂蒋善群张善春戴成祥毛广运王燕方治安臧桐华杨建华王滨燕
Owner ANHUI PROV INST OF BIOLOGICAL MEDICINE
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