Laboratory artificial cultivating method for intertidal zone marine sponge

A breeding method and laboratory technology, applied in the field of artificial cultivation of sponges in the intertidal zone, can solve the problems of low success rate of field cultivation, death of sponges, complicated steps, etc., and achieve simple control of dissolved oxygen concentration, wide application prospects, and complete technology Effect

Inactive Publication Date: 2008-01-09
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of active substances in the sponge is very small, generally only 10% of the wet weight. -8 , "Insufficient supply of drug sources" that is, there is not enough sponge to extract a certain amount of active substances for in-depth research on drugs, making some drugs only stay in the clinical trial stage and cannot continue, thus "Insufficient supply of drug sources" has become a limiting factor that significantly lags behind the development of sponge drugs
A variety of methods have been tried to solve this problem, but there are key technologies that are difficult to solve, such as sponge cell culture technology. At present, no cell line that can be continuously passaged has been established; chemical synthesis is expensive, the steps are complicated, and the actual feasibility is too low; At present, it is generally believed that sponge factory culture is considered to be one of the more promising ways to solve the problem of "insufficient drug source supply".
Most of the foreign reports on sponge culture involve deep-sea sponges. Most of their cultivation of sponge explants is concentrated in field culture in seawater. Metal grids, cement trays or ropes are built in water bodies with a water depth of 5-10m, and the sponges are placed on the ground. After collecting and cutting pieces, fix them on it, but due to the influence of uncertain storms and other weather, sponges often die and are taken away by storms, resulting in a low success rate of field breeding and relatively large uncertainty

Method used

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  • Laboratory artificial cultivating method for intertidal zone marine sponge
  • Laboratory artificial cultivating method for intertidal zone marine sponge
  • Laboratory artificial cultivating method for intertidal zone marine sponge

Examples

Experimental program
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Effect test

Embodiment 1

[0034] 5L static water system culture.

[0035] Hymeniacidon perleve was collected in the intertidal zone of Lingshuiqiao, Dalian, China in July 2005. There are few impurities and dirt on the surface of the sponge. The area selected for collection is about 2×2cm 2 After being brought back to the laboratory, it was placed in a 12L glass tank filled with seawater for temporary raising for 2 days, and the temporary raising temperature was controlled at about 20±1°C. The culture system is shown in Figure 1. At the beginning of cultivation, fix the sponges on a 4×4cm frosted glass carrier with threads, put 15 pieces in total into a 5L glass jar filled with seawater, and then place the glass jar in a water area with a temperature of about 20±2°C, and air pump Air is introduced into the gas distributor, dissolved oxygen meter is used to measure the dissolved oxygen in the water, and the air flow rate is adjusted so that the dissolved oxygen concentration in the water is within the r...

Embodiment 2

[0043] 5L fed-batch system culture. The luxuriant membrane sponge (Hymeniacidon perleve) was collected in the intertidal zone of Lingshuiqiao, Dalian, China in July 2005. After being brought back to the laboratory, it was kept in a 12L glass tank filled with seawater for 2 days, and the temperature was controlled at 18-20°C. The culture system is shown in Figure 2. At the beginning of the cultivation, the sponges were fixed on a circular frosted glass carrier with a diameter of about 5cm, a total of 15 pieces were placed in a 5L liquid liter incubator filled with seawater, the water flow rate was 20L / day, and the outside of the incubator was wrapped with a cooling tube for control The temperature of the culture water body is 20±1°C, the air pump and the gas diffuser provide the oxygen required by the sponge, the whole system is placed indoors in a place where there is no sunlight, and the food is fed with Nitzschia crescentus and Chrysophyllum equilateralis, each at a concentr...

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Abstract

The present invention relates to a method for making intertidal sponge undergo the process of artificial cultivation under the laboratory condition to obtain biomass. Said method includes the following steps: collecting sponge in intertidal zone, cleaning impurity and dirts from sponge surface in laboratory, cutting sponge into small blocks, making temporary culture in culture vessel, then fixing the small block sponge on the carrier, placing it into a culture device to make temperature-controlled photophobic culture, adding bait algae as main body food, making culture for 10-20 days, so that the biomass maxmum value of primary sponge growth can be obtained.

Description

technical field [0001] The invention relates to the technical field of marine organisms, and relates to a technique for producing sponge biomass by adopting a sponge explant culture method in a laboratory, specifically establishing a method for artificially cultivating intertidal sponges in a laboratory. Background technique [0002] Among the currently known marine organisms, sponges are the largest source of active natural products, such as anti-tumor, anti-fungal, and even anti-HIV active natural products, and the probability of finding active compounds obtained from sponges is higher than other animals in the ocean. Plants, so the development of sponge drugs has become a hot spot in the development of marine drugs in recent years. However, the content of active substances in the sponge is very small, generally only 10% of the wet weight. -8 , "Insufficient supply of drug sources" that is, there is not enough sponge to extract a certain amount of active substances for in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00C12N5/06C12N5/07
CPCY02A40/81
Inventor 张卫薛凌云张喜昌靳艳曹旭鹏付晚涛
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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