Laboratory artificial cultivating method for intertidal zone marine sponge

Abstract

The present invention relates to a method for making intertidal sponge undergo the process of artificial cultivation under the laboratory condition to obtain biomass. Said method includes the following steps: collecting sponge in intertidal zone, cleaning impurity and dirts from sponge surface in laboratory, cutting sponge into small blocks, making temporary culture in culture vessel, then fixing the small block sponge on the carrier, placing it into a culture device to make temperature-controlled photophobic culture, adding bait algae as main body food, making culture for 10-20 days, so that the biomass maxmum value of primary sponge growth can be obtained.

Description

technical field [0001] The invention relates to the technical field of marine organisms, and relates to a technique for producing sponge biomass by adopting a sponge explant culture method in a laboratory, specifically establishing a method for artificially cultivating intertidal sponges in a laboratory. Background technique [0002] Among the currently known marine organisms, sponges are the largest source of active natural products, such as anti-tumor, anti-fungal, and even anti-HIV active natural products, and the probability of finding active compounds obtained from sponges is higher than other animals in the ocean. Plants, so the development of sponge drugs has become a hot spot in the development of marine drugs in recent years. However, the content of active substances in the sponge is very small, generally only 10% of the wet weight. -8 , "Insufficient supply of drug sources" that is, there is not enough sponge to extract a certain amount of active substances for in...

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Problems solved by technology

However, the content of active substances in the sponge is very small, generally only 10% of the wet weight. -8 , "Insufficient supply of drug sources" that is, there is not enough sponge to extract a certain amount of active substances for in-depth research on drugs, making some drugs only stay in the clinical trial stage and cannot continue, thus "Insufficient supply of drug sources" has become a limiting factor that significantly lags behind the development of sponge drugs

A variety of methods have been tried to solve this problem, but there are key technologies that are difficult to solve, such as sponge cell culture technology. At present, no cell line that can be continuously passaged has been established; chemical synthesis is expensive, the steps are complicated, and the actual feasibility is too low; At present, it is generally believed that sponge factory culture is considered to be one of the more promising ways to solve the problem of "insufficient drug source supply".

Most of the foreign reports on sponge culture involve deep-sea sponges. Most of their cultivation of sponge explants is concentrated in field culture in seawater. Metal grids, cement trays or ropes are built in water bodies with a water depth of 5-10m, and the sponges are placed on the ground. After collecting and cutting pieces, fix them on it, but due to the influence of uncertain storms and other weather, sponges often die and are taken away by storms, resulting in a low success rate of field breeding and relatively large uncertainty

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