Technique for preparing ricinoleic acid by using combined lipase to hydrolyzing castor oil

A technology of ricinoleic acid and castor oil, applied in the directions of fatty acid production, fatty acid chemical modification, hydrolase, etc., to achieve the effects of improving enzymatic hydrolysis efficiency, good industrial application prospects, and mild reaction conditions

Inactive Publication Date: 2008-01-09
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a process for preparing ricinoleic acid by hydrolyzing castor oil with combined

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Put castor oil and water (10 grams of castor oil) with a molar ratio of 1:1 into a stoppered Erlenmeyer flask and mix evenly, and place in a reciprocating shaker with automatic temperature control and heat to 35°C, then add dry weight The lipase Lipozyme TL of 3% and the lipase Novozym435 of 1% of the oil quality started to react, and the hydrolysis rate of castor oil was 60% after 30 hours, and the hydroxyl value did not change (meaning that the hydroxyl group did not destroy during the reaction). Control experiments show that if any lipase is used alone, its effect will be significantly lower than the combination of two lipases.

Embodiment 2

[0011] Put castor oil and water (10 grams of castor oil) with a molar ratio of 1:5 into a conical flask with a stopper and mix evenly, and place in a reciprocating shaker with automatic temperature control and heat to 50°C, then add dry weight The lipase Lipozyme TL of 5% and the lipase Novozym435 of 2% of oil quality start to react, and the hydrolysis rate of castor oil is 80% after 10 hours, and the hydroxyl value does not change (meaning that the hydroxyl group does not destroy during the reaction).

Embodiment 3

[0013] Castor oil and water (castor oil 10 g) with a molar ratio of 1:50 were put into a stoppered Erlenmeyer flask and mixed evenly, and placed in a reciprocating shaker with automatic temperature control and heated to 45°C, then added dry weight Be the lipase (derived from R.oryzae IFO4697) of 5% grease quality and the lipase Novozym435 of 3% to start reaction, the hydrolysis rate of castor oil is 90% through 20 hours, and hydroxyl value does not change (shows that hydroxyl has no damage occurs).

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Abstract

Production of ricinolic acid from combined lipase hydrolysable castor oil is carried out by: reacting at 35-60 deg. C for 10-30 hrs in proportion of water and castor oil=1-50:1 mol in biochemical reactor, and hydrolyzing. The recovery rate is not less than 90% and hydroxide radical isn't destructed. It's efficient and has less consumption.

Description

technical field [0001] The invention belongs to the field of oil hydrolysis, and in particular proposes a process for preparing ricinoleic acid by combining lipases to hydrolyze castor oil. Different lipases are used to effectively combine and hydrolyze castor oil to prepare ricinoleic acid. The process can effectively protect ricinoleic acid in castor oil. Hydroxyl, while significantly improving the efficiency of enzymatic hydrolysis and the yield of ricinoleic acid, has obvious application prospects. Background technique [0002] The world's annual output of castor oil reaches 1800kt, which is mainly used in the production of coatings, surfactants, medicine, hardened oil, sebacic acid, undecylenic acid, cosmetics and various polymer monomers. Castor oil is mainly composed of triglycerides of higher fatty acids, and 80% to 90% of the fatty acids are ricinoleic acid (9-enyl-12 hydroxyoctadecanoic acid). The saponification value of castor oil is above 178mg KOH / g, and the io...

Claims

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Application Information

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IPC IPC(8): C11C3/00C12S3/18C12N9/20
Inventor 刘德华杜伟
Owner TSINGHUA UNIV
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