Biocontrol bacteria compound agent for preventing and controlling crop soil-infectious diseases and preparation method

A soil-borne disease and biocontrol technology, which is applied in the fields of botanical equipment and methods, plant growth regulators, biocides, etc., can solve the problems of high-efficiency Bacillus subtilis and Trichoderma viride, and the like. Achieving the best medicinal effect, reducing the amount and frequency of use, and the effect of strong colonization ability

Inactive Publication Date: 2008-02-20
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But so far, there is no microbial compound preparation with high-efficiency Bacillus subtilis and Trichoderma vi

Method used

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  • Biocontrol bacteria compound agent for preventing and controlling crop soil-infectious diseases and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Implementation example 1: get 10 grams of crop rhizosphere soil, add sterile water to shake and then gradiently dilute to 10,000 times to make soil suspension, get 1 milliliter of this suspension and add 9 milliliters of PDA medium containing streptomycin sulfate (potato 200 grams of glucose, 20 grams of agar, 20 grams of agar, and 1000 ml of water) to make a plate, culture at 26°C for 48 hours, obtain Trichoderma viride (Trichoderma viride) and Bacillus subtilis (Bacillus subtilis) after separation and purification, and transfer to a test tube The slant is used as the mother fungus for preservation.

[0020] Subtilis and Trichoderma viride used in the medium formulations of the secondary strain fermentation broth are: yeast extract 5 grams, 30 grams of sucrose, 1000 milliliters of water. The production method is as follows: weigh 5 grams of yeast extract and 30 grams of sucrose in a large beaker, add a small amount of water and heat to dissolve completely, then add wat...

Embodiment 2

[0023] Implementation example 2: get 10 grams of crop rhizosphere soil, add sterile water to shake and then gradiently dilute to 10,000 times to make soil suspension, get 1 milliliter of this suspension and add 9 milliliters of PDA medium containing streptomycin sulfate (potato 200 grams of glucose, 20 grams of agar, 20 grams of agar, and 1000 ml of water) to make a plate, culture at 26°C for 48 hours, obtain Trichoderma viride (Trichoderma viride) and Bacillus subtilis (Bacillus subtilis) after separation and purification, and transfer to a test tube The slant is used as the mother fungus for preservation.

[0024] Subtilis and Trichoderma viride used in the medium formulations of the secondary strain fermentation broth are: yeast extract 5 grams, 30 grams of sucrose, 1000 milliliters of water. The production method is as follows: weigh 5 grams of yeast extract and 30 grams of sucrose in a large beaker, add a small amount of water and heat to dissolve completely, then add wat...

Embodiment 3

[0028] Implementation example 3: get 10 grams of crop rhizosphere soil, add sterile water to shake and then gradiently dilute to 10,000 times to make soil suspension, get 1 milliliter of this suspension and add 9 milliliters of PDA medium containing streptomycin sulfate (potato 200 grams of glucose, 20 grams of agar, 20 grams of agar, and 1000 ml of water) to make a plate, culture at 26°C for 48 hours, obtain Trichoderma viride (Trichoderma viride) and Bacillus subtilis (Bacillus subtilis) after separation and purification, and transfer to a test tube The slant is used as the mother fungus for preservation.

[0029] The formula of the medium for the fermentation broth of the secondary strain is: 5 grams of yeast extract, 30 grams of sucrose, and 1000 milliliters of water. The production method is as follows: weigh 5 grams of yeast extract and 30 grams of sucrose in a large beaker, add a small amount of water and heat to dissolve completely, then add water to 1000 ml. The ster...

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Abstract

The invention discloses a biological bacterium prevention compound agent used for preventing and curing a soil-borne disease of the crops, which is characterized in that the contents and weight ratios of all components are: 7 to 9 of dried grass fungus gemma bacillus fermentation broth, 7 to 9 of green wood mold sporule powder, 60 to 80 of diatomaceous earth or light calcium carbonate, 1 to 3 of potassium acid phosphate and 2 to 5 of sodium lignin sulfonate. Through the ferment cultivating to the dried grass fungus gemma bacillus, the fermentation broth is obtained, and the fermentation broth with certain bacterium content is compounded with the green wood mold sporule powder, a microorganism compound agricultural drug is made based on a carrier of diatomaceous earth and by adding suitable nourishing elements. The dried grass fungus gemma bacillus and the green wood mold sporule powder form an effect of mutual predominance compensation. The invention is used for preventing and curing soil-borne diseases, such as a damping off disease, a stand drying disease, a root corrosion disease and a blast which are caused by corrupting leaf mold and fusarium. The product is harmless and is steady and durable in drug effect.

Description

technical field [0001] The invention relates to a biocontrol bacteria compound agent for preventing and treating soil-borne diseases of crops and a preparation method thereof. Background technique [0002] In recent years, with the adjustment of my country's agricultural industry structure, facility agriculture based on greenhouses and greenhouses has developed rapidly. Soil pests infect the roots and stem bases of crops, causing the occurrence of soil-borne diseases such as crop damping, root rot, wilting, and wilting to expand year by year. The continuous cropping of various vegetables, fruits, and flowers in greenhouses and greenhouses all year round, the temperature, humidity, and nutritional conditions of the soil are conducive to the propagation of soil-borne pathogens, and the resulting losses usually reach 30%-40%, or even extinction. Because there are many types of pathogenic bacteria that cause soil-borne diseases, most of which are facultative parasites, have a w...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P3/00A01N59/26A01N61/00
Inventor 纪明山王英姿谷祖敏祁之秋魏松红张杨李兴海
Owner SHENYANG AGRI UNIV
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