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Culture method of edible bolete liquid bactery of Laoshan mount

A technology of liquid strains and culture methods, applied in the direction of fungi, etc., can solve the problems of low yield, Laoshan boletus strains and unsatisfactory liquid culture effects, etc., and achieve good growth and simple formula effects

Inactive Publication Date: 2008-02-27
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Laoshan edible boletus produced in Laoshan, Qingdao, Shandong Province has full fruiting bodies, delicious taste and rich nutrition, but the output is not high due to climate and seasonal conditions. It needs to obtain a large amount of mycelium through fermentation to meet its application in food and other industries. The culture medium and culture condition that have been reported at present are unsatisfactory to the preparation Laoshan boletus bacterial classification and liquid culture effect, therefore urgently need to provide the cultivation method of new Laoshan edible boletus liquid bacterial classification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0034] Wash the fresh potatoes, peel them, cut them into small pieces, and weigh them; measure 1000 ml of distilled water, put them into a large beaker and boil them, add 205 grams of potatoes, 19 grams of dry pine root powder, boil on low heat for 30 minutes, then Filter with two layers of gauze, add 15 grams of agar and 20 grams of glucose to the boiled juice, cook until the agar is completely dissolved and dissolve the solution to 1000 ml, adjust the pH to 4.8 with hydrochloric acid; autoclave at 121°C for 30 minutes. After the sterilization is completed, take it out and cool it to 50°C, add 20 microliters of vitamin B1 mother solution (50mg / ml), mix well, pour it into a plate on an ultra-clean workbench, and use it after solidification.

[0035] Take the fresh Laoshan edible boletus fruiting bodies produced in summer in Shangbei Jiushui and other areas of Laoshan Mountain, carefully cut off the soil and surface dirt at the base of the stalk with a knife, perform aseptic ope...

Embodiment 1-2

[0037] Measure 1000 ml of distilled water, put it into a large beaker and boil it, add 196 grams of potatoes, 21 grams of dried pine root powder, boil for 30 minutes on low heat, then filter with two layers of gauze, add 19 grams of glucose to the boiled juice, and set to volume to 1000 ml, adjust the pH to 5.0 with hydrochloric acid; autoclave at 120°C for 30 minutes. After the sterilization is completed, take it out and cool it down to 50°C, add 20 microliters of vitamin B1 mother solution (50mg / ml), mix well, and pack / dispense into Erlenmeyer flasks.

[0038] According to the above examples, the Erlenmeyer bottle liquid culture medium of selected consumption and quantity can be prepared.

[0039] Take out the bacteria stored in the refrigerated (frozen) equipment, and use a sterile puncher to punch out about 3, 4 or 5 cm on the ultra-clean workbench. 2The bacterium cake was inoculated in 30ml bottled liquid culture medium, 25 ℃, shaking (250r / min) cultivated for 6 days, tr...

Embodiment 2-1

[0043] Wash the fresh potatoes, peel them, cut them into small pieces, and weigh them; measure 1000 ml of distilled water, put them into a large beaker and boil them, add 190 grams of potatoes, 15 grams of dry pine root fine powder, boil on low heat for 30 minutes, then Filter with two layers of gauze, add 12 grams of agar and 18 grams of glucose to the boiled juice, cook until the agar is completely dissolved, dissolve the solution to 1000 ml, adjust the pH to 4.6 with hydrochloric acid; minute. After the sterilization is completed, take it out and cool it to 50°C, add 16 microliters of vitamin B1 mother solution (50mg / ml), mix well, pour it into a plate on an ultra-clean workbench, and use it after solidification.

[0044] Take the fresh Laoshan edible boletus fruiting bodies produced in summer in Shangbei Jiushui and other areas of Laoshan Mountain, carefully cut off the soil and surface dirt at the base of the stalk with a knife, perform aseptic operation on an ultra-clean...

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PUM

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Abstract

The invention discloses a culturing method of Mountain Lao edible liquid bacteria of bolete, which comprises the following steps: using potato, glucose, pine root powder, vitamin B1, agar and distilled water as raw material to dispense culture medium and liquid culture medium according to certain proportion; adopting the culture medium to culture partial fruiting body of Mountain Lao edible liquid bacteria of bolete; fermenting the mycelium according to the liquid culturing method to do rapid liquid ferment and culture for the Mountain Lao edible liquid bacteria of bolete.

Description

technical field [0001] The invention relates to a cultivation method of edible mushroom liquid strains, in particular to a cultivation method of Laoshan edible boletus liquid strains produced in Laoshan, Qingdao, Shandong. Background technique [0002] Delicious boletus belongs to the order Basidiomycetes and the genus Boletus of the family Boletaceae. According to reports, delicious boletus contains more than 20 kinds of amino acids, various minerals, vitamins and polysaccharides, and has the functions of coordinating endocrine and improving human body function. immune function and significant antitumor activity. Liquid culture is the basic method for large-scale production of microbial products. It can obtain a large amount of biological products in a short period of time. This method has the advantages of not being limited by environmental and climatic conditions, good repeatability, easy to scale up the process, and no doubt about the safety. . Because boletus delicios...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 韩怡
Owner QINGDAO UNIV
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