Culture method of edible bolete liquid bactery of Laoshan mount

A technology of liquid strains and culture methods, applied in the direction of fungi, etc., can solve the problems of low yield, Laoshan boletus strains and unsatisfactory liquid culture effects, etc., and achieve good growth and simple formula effects

Inactive Publication Date: 2008-02-27
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Laoshan edible boletus produced in Laoshan, Qingdao, Shandong Province has full fruiting bodies, delicious taste and rich nutrition, but the output is not high due to climate and seasonal conditions. It needs to obtain a large amount of mycelium through fermentation to meet its application in food and other

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example 1-1

[0034] Wash the fresh potatoes, peel them, cut into small pieces, and weigh them; Measure out 1000ml of distilled water, boil in a large beaker, add 205g potatoes, 19g dried pine root powder, and boil for 30 minutes on low heat, then Filter with two layers of gauze, add 15 grams of agar and 20 grams of glucose to the boiling juice, cook until the agar is completely dissolved, and the solution will be dissolved to 1000 ml. Adjust the pH to 4.8 with hydrochloric acid; autoclave at 121°C for 30 minutes. After the sterilization is completed, take it out and cool it to 50°C, add 20 microliters of vitamin B1 mother liquor (50mg / ml), mix well, pour the plate on the ultra-clean workbench, and use it after solidification.

[0035] Take the fresh Laoshan edible bolete fruiting bodies produced in Beijiushui and other areas on Laoshan in summer, carefully cut off the soil and surface dirt at the base of the handle with a knife, and perform aseptic operation on the ultra-c...

Example Embodiment

[0036] Example 1-2

[0037] Measure 1000 ml of distilled water, boil it in a large beaker, add 196 grams of potato, 21 grams of dry pine root powder, boil for 30 minutes on low fire, then filter with two layers of gauze, add 19 grams of glucose to the boiling juice, and make the volume constant To 1000 ml, adjust the pH to 5.0 with hydrochloric acid; autoclave at 120°C for 30 minutes. After the sterilization is completed, take it out and cool to 50°C, add 20 microliters of vitamin B1 mother liquor (50mg / ml), mix well, and pack / dispense into a triangular flask.

[0038] According to the above examples, the selected amount and quantity of liquid culture medium in triangular bottles can be prepared.

[0039] Take out the strains stored in the refrigerated (frozen) equipment, and use a sterile puncher to take about 3, 4 or 5 cm on the ultra-clean workbench 2The bacterial cake was inoculated into 30ml bottled liquid medium, cultured for 6 days at 25℃, shaking (250r / min), transferred to...

Example Embodiment

[0042] Example 2-1

[0043] Wash the fresh potatoes, peel them, cut them into small pieces, and weigh them; Measure out 1000 ml of distilled water, boil in a large beaker, add 190 grams of potatoes, 15 grams of dried pine root powder, and boil for 30 minutes on low heat, then Filter with two layers of gauze, add 12 grams of agar and 18 grams of glucose to the boiling juice. Cook until the agar is completely dissolved. The solution will be dissolved to 1000 ml. Adjust the pH to 4.6 with hydrochloric acid; autoclave at 120, 121 or 130°C for 30 minute. After the sterilization is completed, take it out and cool it to 50°C, add 16 microliters of vitamin B1 mother liquor (50mg / ml), mix well, pour the plate on the clean bench, and use it after solidification.

[0044] Take the fresh Laoshan edible bolete fruiting bodies produced in Beijiushui and other areas on Laoshan in summer, carefully cut off the soil and surface dirt at the base of the handle with a knife, and perform aseptic opera...

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PUM

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Abstract

The invention discloses a culturing method of Mountain Lao edible liquid bacteria of bolete, which comprises the following steps: using potato, glucose, pine root powder, vitamin B1, agar and distilled water as raw material to dispense culture medium and liquid culture medium according to certain proportion; adopting the culture medium to culture partial fruiting body of Mountain Lao edible liquid bacteria of bolete; fermenting the mycelium according to the liquid culturing method to do rapid liquid ferment and culture for the Mountain Lao edible liquid bacteria of bolete.

Description

technical field [0001] The invention relates to a cultivation method of edible mushroom liquid strains, in particular to a cultivation method of Laoshan edible boletus liquid strains produced in Laoshan, Qingdao, Shandong. Background technique [0002] Delicious boletus belongs to the order Basidiomycetes and the genus Boletus of the family Boletaceae. According to reports, delicious boletus contains more than 20 kinds of amino acids, various minerals, vitamins and polysaccharides, and has the functions of coordinating endocrine and improving human body function. immune function and significant antitumor activity. Liquid culture is the basic method for large-scale production of microbial products. It can obtain a large amount of biological products in a short period of time. This method has the advantages of not being limited by environmental and climatic conditions, good repeatability, easy to scale up the process, and no doubt about the safety. . Because boletus delicios...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 韩怡
Owner QINGDAO UNIV
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