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Potassium diagnosis/determination reagent kit and potassium concentration determination method

A determination method and technology of potassium concentration, which is applied in the field of medical/food/environmental inspection and determination, and can solve the problems of expensive electrodes

Inactive Publication Date: 2008-04-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the ion selective electrode method is widely used, but the cost of the electrode is expensive.

Method used

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  • Potassium diagnosis/determination reagent kit and potassium concentration determination method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The potassium diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0043] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0044] Stabilizer 50mmol / L

[0045] Reduced coenzyme 0.25mmol / L

[0046] Tryptophanase 12000U / L

[0047] Lactate dehydrogenase 12000U / L

[0048] Tryptophan 10mmol / L

[0049] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0050] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested potassium sample to the reagent is 1 / 25, The reaction direction is negative reaction (decreasing reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0051] After adding the samples and...

Embodiment 2

[0053] The potassium diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0054] Reagent 1

[0055] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0056] Stabilizer 50mmol / L

[0057] Reduced coenzyme 0.25mmol / L

[0058] Tryptophan 20mmol / L

[0059] Reagent 2

[0060] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0061] Stabilizer 50mmol / L

[0062] Tryptophanase 24000U / L

[0063] Lactate dehydrogenase 24000U / L

[0064] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0065] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the potassium sample to be tested and reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is negative reaction (downward reac...

Embodiment 3

[0068] The potassium diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0069] Reagent 1

[0070] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0071] Stabilizer 50mmol / L

[0072] Reduced coenzyme 0.25mmol / L

[0073] Reagent 2

[0074] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0075] Tryptophan 12mmol / L

[0076] Reagent 3

[0077] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 50mmol / L

[0079] Tryptophanase 30000U / L

[0080] Lactate dehydrogenase 30000U / L

[0081] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0082] When measuring the potassium concentration, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, potassium samp...

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Abstract

The invention relates to a kalium diagnosing / testing reagent box which utilizes the enzyme color method and the enzyme connection method, and also relates to a method for testing the consistence of kalium, and the elements and the components of reagent. The invention belongs to the technical field of the medicine / food / environment check. The reagent box of the invention mainly comprises cushion fluid, reduced coenzyme, hydroxy-tryptophan, hydroxy-tryptophan enzyme, lactic acid dehydrogenase and stabilizer. A series of enzyme reactions occur by mixing the sample and the reagent according to a certain volume rate. Then the reactant is arranged under an ultraviolet / visible light analysis instrument to test the reduction speed of the absorbency at the 340nm of the main wavelength, thereby testing the consistence of the kailium. By adopting the invention, the testing result can be obtained by the ultraviolet / visible light analysis instrument.

Description

technical field [0001] The invention relates to a potassium diagnosis / measurement kit, and meanwhile, the invention also relates to a method for measuring potassium concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] Clinical significance of three decisive levels of serum potassium: below the lower limit of the reference value, showing hypokalemia due to renal tubular disease, hyperaldosteronism, hyperinsulinism, metabolic alkalosis, diuretic therapy, insulin therapy for diabetes During ketosis, potassium ions are transferred into cells, etc. If it is higher than the upper limit of the reference value, it may be caused by glomerular disease, renal insufficiency, diabetic ketosis, adrenal cortical insufficiency, etc. When the serum potassium is as high as 7.5mmol / L or above, the patient will experience arrhythmia, and appropriate treatment should be considered. When serum potassium ex...

Claims

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Application Information

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IPC IPC(8): G01N33/84G01N21/78G01N21/25C12Q1/32C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD