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Lactobacillus acidophilus enriched medium suitable for fermenting soya-bean milk

A technology for fermenting Lactobacillus acidophilus and soybean milk, which is applied in the field of Lactobacillus acidophilus proliferation medium, can solve the problems of complex composition, high cost, and cumbersome production process of the Lactobacillus acidophilus enrichment medium, and achieve low cost and high production efficiency. Strong acid capacity and good cultivation effect

Inactive Publication Date: 2008-05-07
ZHEJIANG GONGSHANG UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0006] The composition of the above-mentioned Lactobacillus acidophilus enrichment medium is too complicated, and the production process is cumbersome; using skim milk as the base medium not only costs a lot, but also the strains grown in the skim milk-based enrichment medium are not suitable for Fermented soymilk, the medium is not suitable for use as a starter for fermented soymilk

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Soymilk preparation: Weigh a certain amount of soybeans with bright color, uniform grain size and full grains, wash them with pure water at least three times to remove the dust and microorganisms attached to the soybean surface, soak them in pure water more than twice the volume of soybeans for 8-14 hours, Soybean cell structure is softened; soaked soybeans are blanched with boiling water for 15 minutes; according to the ratio of soybeans (before soaking): water = 1: 6 (mass / volume), add hot water at 80°C, and grind with a refiner. Obtain coarse soybean milk; filter the coarse soybean milk with eight layers of gauze, and finally obtain soybean milk.

[0018] Preparation of other components: tomato juice, tomato juice, beating after washing, adding water to boil at a ratio of 1:1, and filtering to obtain tomato juice; corn extract juice, mixing corn flour with water at a ratio of 1:2, and bathing in a constant temperature water bath at 60°C for 1 hour Then filter to obta...

Embodiment 2

[0029] The preparation of soymilk and other components of the culture medium were the same as in Example 1.

[0030] Preparation of enrichment medium:

[0031] Soymilk 1000ml

[0032] Glucose 20g

[0033] Tomato Juice 150mL

[0034] Corn Extract 90mL

[0035] Carrot Juice 50mL

[0036] Pleurotus ostreatus extract juice 60mL

[0037] Initial pH=5.52, sterilization conditions: 116°C autoclaving for 15 minutes.

[0038] Inoculate Lactobacillus acidophilus (preservation number: CGMCC No.2122) in the enrichment medium according to the inoculum amount of 1%, cultivate it at 37°C for about 10 hours, and the strain enters the growth equilibrium period, and the number of viable bacteria at this time can reach 1.45× 10 9 cfu / mL, the pH of the culture was 3.71.

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Abstract

The invention relates to a lactobacillus acidophilus multiplication medium, in particular to the lactobacillus acidophilus multiplication medium for soybean milk fermentation. The soybean milk is used to be a basic culture medium; a plurality of tomato juice, corn extract juice, carrot juice, oyster mushroom extract juice and other proliferation factors are added into the soybean milk to produce the lactobacillus acidophilus multiplication medium, in which the lactobacillus acidophilus strain can be cultivated in thirty seven degree centigrade, and ten hours later, the active fungus quantity can exceed over 109cfu / mL. The invention is suitable for making the starter of soybean milk fermentation, providing the soybean milk fermentation with the fermentation seed fungus of exuberant growth ability, high active fungus quantity and strong acid producing ability.

Description

technical field [0001] The invention relates to a proliferating medium for Lactobacillus acidophilus, in particular to a proliferating medium for Lactobacillus acidophilus suitable for fermenting soybean milk. Background technique [0002] Lactobacillus acidophilus belongs to the genus Lactobacillus and ferments carbohydrates to produce large amounts of lactic acid. It widely exists in the intestinal tract of humans and animals, and plays an important role in the health of the human body, especially in maintaining the normal physiological functions of the gastrointestinal tract. It is an important beneficial microbial flora in the intestinal tract. [0003] Only when Lactobacillus acidophilus reaches a certain amount can it improve and regulate the balance of the microbial flora in the host intestine and promote the health of the host. [0004] Due to the special requirements for the growth conditions of Lactobacillus acidophilus, the live bacteria content of Lactobacillus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L1/20C12R1/23A23L11/00
Inventor 孟岳成范兴艺姜涛
Owner ZHEJIANG GONGSHANG UNIVERSITY
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