Human papillomavirus gene parting detecting reagent case and method for preparing gene chip thereof

Abstract

The invention discloses a probe with a nucleotide sequence complementary to human papilloma virus HPV DNA, a genotyping detection kit containing the probe and a gene chip preparation method thereof. The probe is selected from SEQ ID Nos: 1-21 and sequences complementary to SEQ ID Nos: 1-21. The kit includes a gene chip with at least one of the above-mentioned probes, a DNA sequence marked with biotin spots, a DNA sequence coding a part of β-globulin, and various primers selected from MY09 / MY11. The gene chip of the kit is prepared through the steps of preparing DNA probes, fixing the probes, using sodium hydroxide to stop the reaction, and the like. The kit of the invention can quickly and accurately detect HPV infection and identify specific subtypes, is more suitable for Chinese people, and has great significance for early diagnosis, prevention, treatment and follow-up of cervical cancer.

Description

technical field

[0001] The invention relates to a probe with a nucleotide sequence complementary to human papillomavirus HPV DNA, a genotyping detection kit for detecting specific subtypes of HPV containing the probe and a gene chip preparation method thereof. Background technique

[0002] Cervical cancer is one of the most common malignancies in women. There are about 500,000 new cases of cervical cancer in the world every year, about 380,000 in Asia, and about 150,000 in China. About 230,000 women die of cervical cancer in the world every year, about 190,000 in Asia, and about 80,000 in China. Cervical cancer is the second most common malignant tumor in women. In particular, human papillomavirus infection has led to an increasing incidence of cervical cancer and precancerous lesions and tends to be younger, which seriously threatens the physical and mental health of women. Therefore, the prevention, diagnosis and treatment of cervical cancer is an urgent problem to be sol...

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