Discovery of forage grass attached lactobacillus plantarum and enterococcus mundtii and use thereof

A technology of Lactobacillus plantarum and Enterococcus montelii, applied in the field of lactic acid bacteria, can solve the problem of rare lactic acid bacteria, and achieve the effect of improving palatability and reducing the loss of nutrients

Inactive Publication Date: 2008-07-23
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In China, there are few reports on the isolati

Method used

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  • Discovery of forage grass attached lactobacillus plantarum and enterococcus mundtii and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0021] Growth experiment of Enterococcus monsonii S1518 at 45°C

[0022](1) Medium used: GYP calcium carbonate agar medium.

[0023] (2) Inoculation: Dip the inoculation needle into the culture medium to be measured that has been grown in the GYP liquid medium, and puncture the inoculation.

[0024] (3) Culture conditions and time: culture at 45°C for 24h-72h to observe the growth.

[0025] (4) Observation of the result: a zona pellucida was formed around the puncture hole.

[0026] This indicated that E. monsonii S1518 was still growing at 45°C.

Embodiment II

[0028] Acid production experiment of Lactobacillus plantarum S2409

[0029] (1) Medium used: GYP culture medium.

[0030] (2) Inoculation method and culture time: inoculate a loop of Lactobacillus plantarum S2409 in GYP liquid medium, and culture at 30° C. for 72 hours.

[0031] (3) Result detection: the pH value of the culture solution was detected to be 3.45 with a portable acidity meter.

[0032] This indicated that Lactobacillus plantarum S2409 could still grow in a higher acidic environment.

Embodiment III

[0034] Dilute 5ml of Lactobacillus plantarum S2409 culture solution and Enterococcus monsonii S1518 culture solution with water, and spray evenly on grass silage raw materials with a watering can. The spraying amount is 10ml / kg, and the number of added bacteria is about 1 ×10 5 cfu, fully stirred and mixed; the control group was sprayed with the same amount of pure water. By sampling and analyzing the silage at different times, the pH value changes are shown in the following table: pH value change table

[0035]

[0036] In conclusion, it can be seen from the table that the pH values ​​of the control group and the addition group are significantly different, and the addition of Lactobacillus plantarum S2409 group and Enterococcus monsonii S1518 group can quickly reduce the pH value of silage at the initial stage of silage; Stable lactic acid fermentation was carried out in the whole process of silage fermentation; Enterococcus monsonii dominated the rapid drop of pH in the ...

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Abstract

The invention relates to two lactobacillus separated from gramineous forage grass, tests later showed, one is Lactobacillus plantarum, and the other is Enterococcus mundtii. Adding both lactobacillus into forage grass for silage, can rapidly reduce the pH value of the forage grass silage, improve the ferment quality of the forage grass, and make the nutritional ingredient of the forage grass be better preserved.

Description

technical field [0001] The invention relates to two strains of lactic acid bacteria that function in grass silage, one is Lactobacillus plantarum S2409 and the other is Enterococcus mundtii S1518. Specifically, the present invention describes the discovery of Lactobacillus plantarum S2409 and Enterococcusmundtii S1518 in grasses and their application in grass silage. Background of the invention [0002] Nowadays, with the development of forage processing industry, the roughage of ruminant livestock has been converted from green grass or hay to a silage production system with labor-saving, high production performance, low cost and available all year round. The technology of producing high-quality silage for this reason has more and more attracted extensive attention of the animal husbandry circle. During the silage preparation process, due to the different dry matter content, carbohydrates, buffer energy, and the number and composition of the bacteria carried in the silage, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/31A23K3/03C12R1/25
Inventor 桂荣刘晗璐塔娜那日苏
Owner GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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