Method for producing fish scale collagen by employing preservative agent

A fish scale collagen and preservative technology, applied in the direction of fermentation, etc., can solve problems such as the inability to effectively control the reproduction of microorganisms, the decline in the basic quality of products, and the corruption of feed liquids, and achieve the effects of shortening the method time, stabilizing the molecular weight, and avoiding corruption

Inactive Publication Date: 2008-07-23
YANTAI DONGCHENG PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such a method will often result in the inability to effectively control the reproduction of microorganisms in the process of the method, resulting in the corruption of the feed liquid, and the result is a decline in the basic quality of the product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1, a method for producing fish scale collagen using preservatives, is to use the prior art to treat fish scale acid and enzymolyze, and add preservatives to the solution obtained after enzymolysis at a temperature of 50-55 ° C, and let it stand for 30 Minutes, then decolorization, ultrafiltration, sterilizing filtration and spray drying. In this embodiment, 100 kilograms of fish scales are dropped into a reaction tank. After decalcification with industrial grade hydrochloric acid, adjust the pH to 7.0-7.5 with NaOH solution and wash with water. ; Add 1000 liters of purified water to the washed fish scales, boil for 30 minutes; lower the temperature to 55-65 ° C, control the pH 6.5, add 1 kg of neutral protease Neutrase 0.8 L, enzymolysis 1-4 hours, enzymolysis After the end, the temperature is raised to 70-90°C and kept for 30 minutes; the solution is cooled to 50-55°C, and preservatives are added. The preservatives used are trehalose, mannan, earthworm extract, ...

Embodiment 2

[0013] Embodiment 2, a kind of production method that adopts antiseptic to produce fish scale collagen protein is to put 100 kilograms of fish scales into reaction tank, after decalcifying with industrial grade hydrochloric acid, adjust pH 7.0-7.5 with NaOH solution, wash with water; Add 300 liters of purified water to the fish scales, heat up and boil for 30 minutes; lower the temperature to 55-65°C, control the pH to 6.5, add 0.1 kg of compound protease Flavorzyme 500L, enzymatically hydrolyze for 1-4 hours, and heat up to 70- 90°C, keep warm for 30 minutes; cool down the solution to 50-55°C, add 2.5-7 kg / ton preservative trehalose, let stand for 30 minutes and then filter; add 3 kg of activated carbon, stir at a temperature of 55-70°C Adsorption for 10-30 minutes; decarbonization and filtration; filter the filtered liquid with an ultrafiltration machine to intercept components with a molecular weight cut-off of 1000-8000 Daltons and concentrate to a solid content of 20%-50%,...

Embodiment 3

[0014] Embodiment 3, a kind of production method that adopts preservative to produce fish scale collagen is that 100 kilograms of fish scales are put into the reaction tank and decalcified with industrial-grade hydrochloric acid, then adjust the pH to 7.0-7.5 with NaOH solution, and wash with water; Add 300 liters of purified water to the fish scales, heat up and boil for 30 minutes; lower the temperature to 55-65°C, control the pH to 6.5, add 0.1 kg of compound protease Flavorzyme 500L, enzymatically hydrolyze for 1-4 hours, and then heat up to 70-90 ℃, keep warm for 30 minutes; cool the solution to 50-55 ℃, add 15-30 g / ton preservatives natamycin and sodium diacetate, let it stand for 30 minutes and filter; add 8 kg of activated carbon, at a temperature of 55 Stir and adsorb at -70°C for 10-30 minutes; decarbonize and filter; filter the filtered liquid with an ultrafiltration machine to intercept components with a molecular weight of 1000-8000 Daltons and concentrate to a sol...

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PUM

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Abstract

The invention discloses a preparation method for fish-scale collagen with preservative, which is characterized in that the fish-scale collagen is obtained from the fish-scale via acid treatment, enzymolysis, adding antiseptic, decoloration, filtration, hyperfiltration and spray drying. The fish-scale collagen with preservative has the advantages of making the fish-scale profitable, simple method and steps, easy operation, low and stable product molecular weight, being without fishy smell and bitterness, very low ash content, high productivity, mild additive antiseptic without poison or side effect and completely eliminating after achieving antiseptic effect; and not affecting the final product quality.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for producing fish scale collagen by using a preservative. Background technique [0002] Collagen accounts for 30-40% of the total protein in the human body, and is the most abundant protein in the body; its amino acid composition is very special, of which glycine (Gly) accounts for 30%, proline (Pro) and hydroxyproline (Hyp) totals about 25%. [0003] Generally, preservatives are not added in the method of enzymatic hydrolysis to extract fish scale collagen, the purpose is to produce products without preservatives to improve product quality. However, such methods often result in the inability to effectively control microbial reproduction in the process of the method, resulting in the corruption of the feed liquid, and the result is a decline in the basic quality of the product. Contents of the invention [0004] The object of the present in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 由守谊齐东绮张立勇曹红光
Owner YANTAI DONGCHENG PHARMA GRP
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