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Method for extracting and purifying two kinds of taxane compound from yew branches and leaves

A technology of yew branches and leaves and acetyl-paclitaxel, which is applied in the direction of organic chemistry, can solve the problems of polluting the environment and wasting plant resources, achieve high-efficiency utilization, realize industrial production, and the effect of simple and easy method

Inactive Publication Date: 2010-12-22
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Taxanes with xylosyl attached at the C-7 position were originally isolated by Senilh, mainly including: 7-xylosyl-10-deacetyl paclitaxel, 7-xylosyl-10-deacetyl tri Pimemannine, 7-xylosyl-10-deacetyl paclitaxel C, etc. These compounds are often discarded as waste in the traditional production process of extracting paclitaxel, which not only wastes plant resources, but also pollutes the environment

Method used

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  • Method for extracting and purifying two kinds of taxane compound from yew branches and leaves
  • Method for extracting and purifying two kinds of taxane compound from yew branches and leaves

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Embodiment 1

[0021] Weigh 200g of southern yew branches and leaves, dry and pulverize, add 2000mL of water for negative pressure cavitation extraction, filter after 30min, and add the filtrate to a glass column equipped with 35g of AB-8 resin for dynamic adsorption. The loading solution is 15BV, The concentrations of 10-deacetylbaccatin III and 7-xylosyl-10-deacetylpaclitaxel in the sample solution were: 0.0657mg / mL and 0.1681mg / mL, the flow rate was 1mL / min, and the temperature was 35 ℃, using gradient elution method: 3BV of 30% ethanol was eluted to remove impurities, and then 6BV was eluted with 80% ethanol, the fractions were collected and concentrated to dryness. The content of -xylosyl-10-deacetyl paclitaxel was increased from 0.2% and 0.053% to 1.69% and 3.34%, respectively, which was increased by 8.54 times and 62.43 times respectively. After the resin was enriched, the sample was purified by 300-400 mesh medium-pressure silica gel column chromatography. The silica gel used was 40 ...

Embodiment 2

[0023]Weigh 200g of Taxus chinensis branches and leaves, dry and pulverize them, add 2000mL of water for negative pressure cavitation extraction, filter after 30min, add the filtrate to a glass column filled with 35g of AB-8 resin, and perform dynamic adsorption. The loading solution is 15BV. The contents of 10-deacetylbaccatin III and 7-xylosyl-10-deacetyl paclitaxel in the sample solution were 0.0519mg / mL and 0.1673mg / mL respectively, the flow rate was 1mL / min, and the temperature was 35°C , using the method of gradient elution: 30% ethanol elution of 3BV to remove impurities, then 6BV 80% ethanol elution, collected fractions, concentrated to dryness, after resin enrichment, 10-deacetylbaccatin III and 7- The content of xylosyl-10-deacetyl paclitaxel increased from 0.16% and 0.049% to 1.57% and 3.46%, respectively, increasing 9.81 times and 70.61 times respectively. After the resin was enriched, the sample was purified by 300-400 mesh medium-pressure silica gel column chroma...

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Abstract

The invention relates to a method for using specific resin and silicon gel column chromatography purification via a negative pressure cavitation water extraction method to produce 10-deacetylbaccatin III and 7-xylose-10-deacetylpaclitaxel at high efficiency. The method comprises using taxus branch leaves as raw material, drying and breaking the raw material to process negative pressure cavitationwater extraction, filtering, using AB-8 resin to process dynamic absorption on filter liquor to concentrate 10-deacetylbaccatin III and 7-xylose-10-deacetylpaclitaxel and carrying out a further purification by middle pressure silicon gel column chromatography, to obtain the 10-deacetylbaccatin III of 60% purity or more and 7-xylose-10-deacetylpaclitaxel of 67% purity or more. The invention can use renewable taxus branch leaves to protect ecological resource and effectively extract effective component and biological semi synthesis precursor materials. The invention has simple operation, thus is suitable for industrial application and is important for industrial production.

Description

technical field [0001] The invention relates to a method for extracting and purifying 10-deacetylbaccatin III and 7-xylosyl-10-deacetyl paclitaxel from branches and leaves of Taxus chinensis. Background technique [0002] Paclitaxel (paclitaxel, figure 1 ) is a diterpenoid compound with a pentamethylpentadecene skeleton, which is a natural anticancer drug extracted from the bark of Pacific yew (Taxus brevifolia) for the first time (Wani et al., 1971). Binding to tubulin, promoting its polymerization, inhibiting the mitosis of cancer cells, and preventing the proliferation of cancer cells (Kelling et al., 2003; She Kun et al., 2001) was officially approved by the US FDA in 1992 as a new anticancer drug. , breast cancer, and lung cancer have a better effect. Studies in recent years have shown that paclitaxel also has certain potential in the treatment of other diseases, such as malignant melanoma (Roshid et al., 1994), rheumatoid arthritis (Oliver et al., 1994) and Alzheimer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D305/14
Inventor 付玉杰祖元刚孙蕊李双明王莹姜守刚汪琢张琳刘威
Owner NORTHEAST FORESTRY UNIVERSITY
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