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Method for preparing gene transmission vector nano calcium phosphate

A technology of calcium phosphate nanometer and delivery carrier, which is applied in gene therapy, chemical instruments and methods, and medical preparations containing active ingredients, etc. It can solve the problem of short gene expression time, low transfection efficiency, and easy phagocytosis by the reticuloendothelial system and other issues to achieve good biocompatibility

Inactive Publication Date: 2008-08-13
张桂英 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, liposomes and cationic polymers mediate the specificity and targeting of gene transfer tissues, the transfection efficiency is low and they are easily phagocytized by the reticuloendothelial system, and the gene expression time is short

Method used

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  • Method for preparing gene transmission vector nano calcium phosphate
  • Method for preparing gene transmission vector nano calcium phosphate
  • Method for preparing gene transmission vector nano calcium phosphate

Examples

Experimental program
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Embodiment Construction

[0010] With 0.05mol / L CaCl 2 : solution and microenvironment (1%SDS 5ml, 1%F-68 5ml, n-butanol, water etc.), room temperature reaction and stirring (300rpm) 24h, form liquid A, then 0.025mol / L Na 2 HPO 4 , 0.025mol / L sodium citrate (Sodium Citrate) solution and microenvironment (1%SDS 5ml, 1%F-68, 5ml, water etc.), room temperature reaction and stirring (300rpm) 24h, form liquid B; After 24 hours Drop liquid B into liquid A at a rate of 10 ml per hour, stir and mix at 350 rpm, control the temperature at 35°C, stir thoroughly for 48 hours, (3) resuspend with 10 ml of acetone and 25 ml of ethanol, and centrifuge at 13500 rDm for 25 min , at room temperature (25°C), washed, ultrasonically dispersed for 1 h, and freeze-dried to obtain an average particle diameter of 23.5-31.4 nm calcium phosphate nanometers.

[0011] See attached picture for pictures.

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Abstract

The present invention provides a preparing method of gene delivery carrier calcium phosphate nanometer grain, the characteristics is individual mixing calcium chloride with disodium hydrogen phosphate or surface active agent by the chemical synthetic method, slow mixing the upper two material and blending via strong blending, calcium phosphate nanometer grain obtained by washing product use ethanol and water, dispersing by ultra audible sound and freeze-dry after reaction time. The invention has a simple process, lower cost and easy to industrialization; the preparing calcium phosphate nanometer grain has positive electrical charge, high efficiency, innocuity and biodegradation; the nanometer grain prepared by the method is evenly distribution, the diameter is mostly distrubited between 23.5-31.4, it is one of an ideal gene delivery carrier.

Description

Technical field: [0001] The nanometer prepared by the method is non-toxic, has good biocompatibility, is biodegradable, can be used as a gene transfer carrier, and is suitable for the fields of biopharmaceuticals and tumor gene therapy. Background technique: [0002] Improving the transformation efficiency and targeting of DNA is the key to gene therapy. Many vectors, viral vectors and non-viral vectors have been widely used in the past. Viral vectors mainly include adenovirus, retrovirus, adeno-associated virus, etc.; but viral vectors are potentially dangerous in terms of safety; and immunogenicity Relatively strong, it will be rejected by the body's immune system soon after injection into the body. For example, when a high concentration of adenovirus vector is injected intravenously, severe inflammation will occur in the liver; non-viral vectors are currently commonly used such as liposomes and polymers Cationic polymers; polycationic polymers include polyamino acids, po...

Claims

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Application Information

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IPC IPC(8): C01B25/32A61K47/02A61K48/00A61K31/7088
Inventor 张桂英刘霆陈永恒
Owner 张桂英
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